Phosphoserine antibody [PSR-45] (ab6639)

Product Name 

Phosphoserine antibody [PSR-45]

Product type 

Primary antibodies

Description 

Mouse monoclonal [PSR-45] to Phosphoserine

Immunogen 

phospho-serine conjugated to KLH
BLAST 'phospho-serine' with or

Reacts with 
(species key)

Hu, Rat, Dm

Specificity 

Monoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH.
No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.

Tested applications 
(see key)

I-ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
I-ELISA: 1/4000.
WB: 1/500.

Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: rat brain cortex lysate
ELISA: phosphoserine conjugated to BSA

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

PSR-45

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 5.20 - 6.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Phosphoserine antibody [PSR-45] images, references, products related to ab6639 and other tools.

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References for Phosphoserine antibody [PSR-45] (ab6639)

  This product has been used in: (two most recent references)

James MK et al. Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol 28:498-510 (2008). WB. PubMed: 17908796

Moya A et al. Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization. J Biol Chem 283:25475-84 (2008). WB. PubMed: 18617510

See all 6 publication references for this product.


If you publish research using ab6639 please let us know so that we can cite the reference on this datasheet.

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