Feedback Form

Western blot - Phosphoserine antibody [PSR-45] (ab6639)
Western blot - Phosphoserine antibody [PSR-45] (ab6639)
(enlarge)


Product Name 

Phosphoserine antibody [PSR-45]

 

See all Phosphoserine antibodies (7)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [PSR-45] to Phosphoserine

Immunogen 

phospho-serine conjugated to KLH
BLAST 'phospho-serine' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Rat, Dm

Specificity 

Monoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH.
No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.

Tested applications 
(see key)

I-ELISA, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• WB

7 Abreviews

- average rating: 3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)


WB
(read Abreview)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
I-ELISA: 1/4000.
WB: 1/500.

Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: rat brain cortex lysate
ELISA: phosphoserine conjugated to BSA

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

PSR-45

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 5.20 - 6.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Phosphoserine antibody [PSR-45] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Phosphoserine antibody [PSR-45] images, references, products related to ab6639 and other tools.

 

Phosphoserine antibody [PSR-45] images:

Product image  Western blot - Phosphoserine antibody [PSR-45] (ab6639)

Western blot - Phosphoserine antibody [PSR-45] (ab6639)

Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg

Secondary
Sheep anti mouse (HRP) at 1/2000 dilution

Performed under reducing conditions.

This image is an edited version of an image submitted courtesy of an Abreview submitted on 17 August 2005. We do not have any further information relating to this image.

See Abreview

 

References for Phosphoserine antibody [PSR-45] (ab6639)

Specific publication references   This product has been used in: (two most recent references)

James MK et al. Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol 28:498-510 (2008). WB. PubMed: 17908796

Moya A et al. Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization. J Biol Chem 283:25475-84 (2008). WB. PubMed: 18617510


See all 6 publication references for this product.


If you publish research using ab6639 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Phosphoserine

 

Phosphoserine antibody [PSR-45] - more information

Customer reviews (feedback) regarding Phosphoserine antibody [PSR-45]

Customer FAQs regarding Phosphoserine antibody [PSR-45]

Protocols for Phosphoserine antibody [PSR-45]

Price and availability of products related to Phosphoserine antibody [PSR-45]

 

Phosphoserine antibody [PSR-45] - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for Phosphoserine antibody [PSR-45]

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other Phosphoserine Antibodies

See all Phosphoserine antibodies (7)...

 

Phosphoserine antibody [PSR-45] - other tools:

Contact Abcam with a Technical Enquiry about Phosphoserine antibody [PSR-45]

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.