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Anti-Phosphoserine antibody [PSR-45] (ab6639)

Abreviews (5)Q&A (24)Specific References (8)

Overview

  • Product nameAnti-Phosphoserine antibody [PSR-45]See all Phosphoserine primary antibodies ...
  • Description
    Mouse monoclonal [PSR-45] to Phosphoserine
  • SpecificityMonoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.
  • Tested applicationsIP, Indirect ELISA, WB more details
  • Immunogen

    phospho-serine conjugated to KLH

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlWB: rat brain cortex lysate ( a main 50kDa band is seen by detection with biotin-avidin AEC staining) ELISA: phosphoserine conjugated to BSA

Properties

Applications

Our Abpromise guarantee covers the use of ab6639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: 1/2000.
Indirect ELISA I-ELISA: 1/4000.
WB WB: 1/500 - 1/1000. Can be blocked with Phosphoserine protein (Active) (ab100812). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Stronger blocking agent percentage is recommended (e.g. 10%), in addition to varying loading amounts and shorter incubation time (1-2h RT)

Target

  • RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
      phospho-Ser antibodypS antibodypSER antibody

Anti-Phosphoserine antibody [PSR-45] images

  • Western blot - Phosphoserine antibody [PSR-45] (ab6639)
    Western blot - Phosphoserine antibody [PSR-45] (ab6639)
    Anti-Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg

    Secondary
    Sheep anti mouse (HRP) at 1/2000 dilution

    Performed under reducing conditions.

    See Abreview

  • Immunoprecipitation - Anti-Phosphoserine antibody [PSR-45] (ab6639)
    Immunoprecipitation - Anti-Phosphoserine antibody [PSR-45] (ab6639)Image courtesy of an anonymous Abreview.
    ab6639 used in Immunoprecipitation.

    Whole cell lysate prepared from murine mammary cells was loaded at 250µg.
    Cells were treated with 5ng/ml TGF beta for varying time periods.
    Protein A was used for the Immunoprecipitation step.
    ab6639 used at a 1/2000 dilution for 10 hours at 4°C.
    Western blot was performed with an HRP anti-PCBP1 polyclonal antibody, 1/10000 dilution.

    See Abreview

References for Anti-Phosphoserine antibody [PSR-45] (ab6639)

This product has been referenced in:
  • Uckun FM  et al. Serine phosphorylation by SYK is critical for nuclear localization and transcription factor function of Ikaros. Proc Natl Acad Sci U S A 109:18072-7 (2012). WB, IP ; Human . Read more (PubMed: 23071339) »
  • Bodiga S  et al. Enhanced susceptibility to biomechanical stress in ACE2 null mice is prevented by loss of the p47phox NADPH oxidase subunit. Cardiovasc Res : (2011). WB . Read more (PubMed: 21285291) »

See all 8 Publications for this product

Publishing research using ab6639 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab6639 Submit a Question for ab6639

Displaying 1 - 10 of 29 results for Abreviews and Q&A

10
Votes: Highest First

we would like to replace the antibodies with the rabbit polyclonal anti-phopshoserine and anti-phosphothreonine antibodies, specifically:

ab9332 anti-phosphoserine rabbit polyclonal
ab9337 anti-phosphothreonine rabbit polyclonal

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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My PI informed me of the idea that she had regarded using 10% BSA in TBS + 0.1% Tween-20 instead of PBS for the possibility that the pSer and pThr antibodies are cross-reacting with the PBS. Unfortunately after attempting this multiples times with various...

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Thank you for your reply.
I am sorry to hear that the suggestions we discussed did not improve the results.

Yes, I'd be happy to replace, credit or refund the antibodies. Please just let me know which option is best for you. In case of a repl...

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As I was stewing over our problems with the phospho ser Abs, it occurred to me just now, that the student was using PBS. Is the phosphate interfering and we should be using TBS? That would be a sweet solution to the problem.
Thoughts?

Thank you for your emails and the additional details you sent.

Yes, the use of PBS is a good point. In case of using anti-phospho antibodies this can actually cause high background due to the presence of phosphate in the buffer. I would suggest...

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Product code: 79851
Lot number: GR23196-1
Inquiry: Please see attached word file. Thank you!

Thank you for contacting us.

I am sorry to hear that the 2 antibodies have not been working as expected.

Your protocol looks fine in general. However, both antibodies are directed against ANY phospho-site on a serine or threonine of a...

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Would this detect in human?

Thank you for your telephone enquiry last week.

I am sorry to confirm that ab6639 Phosphoserine antibody [PSR-45] has only been tested in rat brain tissue bythe originator, and so we have no images for human samples.

There have, howe...

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Dear Sir or Madam,   We would like to use ab6639 for IHC.  I don’t see that in the tested applications.  Has it been tested and shown not to work for IHC or has it not been tested for that?  Thanks for your help. 

Thank you for contacting us.

To our knowledge this product has not been tested in IHC. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discoun...

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Thank you. The PO # is ******, ordered on *****. A refund would be great, as I am currently testing another a-PSER antibody that was recommended. If it does not work well enough, I may try a different abcam one.
Let me know if you need any other info...

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Thank you for your reply and providing that information.

Your credit note ID is ********.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish t...

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Inquiry: I am trying to detect serine phosphorylation of a protein that I am IP'ing. However, I cannot get the suggested positive control to work (mouse brain lysate). Protein Loading: 30ug Semi-dry transfer onto PVDF membrane (always dipped in...

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Thank you for contacting Abcam.

Based on the information that you have provided, I do not think that there is any protocol tips that would help resolve the issue, as I think you have tried everything.

Under our Abpromise, which is good...

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Après réflexion nous souhaiterions plutôt bénéficier d'un avoir ou d'un remboursement de l'Anticorps anti-phosphoSerine [PSR-45] (Commande #*******). Pourriez-vous m'indiquer les modalités et le délai de la ...

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Merci pour votre email.

J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est ******.

Voici les manières dont vou...

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i have used ab6639 to detect phosphoserine from endocytic vesicle extract from rat liver samples. however a short 1 min exposure produces no staining and a 3 min exposure produces a totally black membrane. I have tried 10% BSA w/ and without 0.1% gelatin ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab76016. To check the status of the order please contact our Customer Se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"