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Anti-Phosphoserine antibody [PSR-45] (ab6639)

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Overview

Product name

Anti-Phosphoserine antibody [PSR-45]
See all Phosphoserine products (8) ...

Description

Mouse monoclonal [PSR-45] to Phosphoserine

Specificity

Monoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.

Tested applications

IP, Indirect ELISA, WBmore details

Immunogen

phospho-serine conjugated to KLH

phospho-se rine

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

WB: rat brain cortex lysate ( a main 50kDa band is seen by detection with biotin-avidin AEC staining) ELISA: phosphoserine conjugated to BSA

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

PSR-45

Isotype

IgG1

Light chain type

unknown

Research areas

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids

  • Western blot - Phosphoserine antibody [PSR-45] (ab6639)Western blot - Phosphoserine antibody [PSR-45] (ab6639) image (enlarge)

  • Immunoprecipitation - Anti-Phosphoserine antibody [PSR-45] (ab6639)Immunoprecipitation - Anti-Phosphoserine antibody [PSR-45] (ab6639) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Alternative names

  • phospho-Ser antibody
  • pSER antibody

Anti-Phosphoserine antibody [PSR-45] images:

  Western blot - Phosphoserine antibody [PSR-45] (ab6639)

Western blot - Phosphoserine antibody [PSR-45] (ab6639)

Anti-Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg

Secondary
Sheep anti mouse (HRP) at 1/2000 dilution

Performed under reducing conditions.

This image is an edited version of an image submitted courtesy of an Abreview submitted on 17 August 2005. We do not have any further information relating to this image.

See Abreview

  Immunoprecipitation - Anti-Phosphoserine antibody [PSR-45] (ab6639)

Immunoprecipitation - Anti-Phosphoserine antibody [PSR-45] (ab6639)

ab6639 used in Immunoprecipitation.

Whole cell lysate prepared from murine mammary cells was loaded at 250µg.
Cells were treated with 5ng/ml TGF beta for varying time periods.
Protein A was used for the Immunoprecipitation step.
ab6639 used at a 1/2000 dilution for 10 hours at 4°C.
Western blot was performed with an HRP anti-PCBP1 polyclonal antibody, 1/10000 dilution.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-Phosphoserine antibody [PSR-45] (ab6639)

This product has been referenced in:

  • Bodiga Set al. Enhanced susceptibility to biomechanical stress in ACE2 null mice is prevented by loss of the p47phox NADPH oxidase subunit. Cardiovasc Res : (2011). WB.Read more (PubMed: 21285291) »
  • Nguyen KAet al. Modulation of gonadotropin-releasing hormone-induced extracellular signal-regulated kinase activation by dual-specificity protein phosphatase 1 in LbetaT2 gonadotropes. Endocrinology 151:4882-93 (2010). WB.Read more (PubMed: 20685880) »

See all 7 publications for this product

Publishing research using ab6639? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"