Anti-Phosphoserine antibody (ab9332)

Thanks a lot for your detail information, which is very helpful. I have considered all product you suggested based on my plan. I think I will buy the  Pan-specific phosphoserine antibody  (ab9332) as you mentioned. The first application is that I will use it to detect a purified protein phosphorylation by GSK3b after kinase assay by  western blot. The purified protein is mouse origin. If it works well, I will apply this antibodies in other experiments in vivo by using immunostaining in different cell line and tissues. The cells and tissues come from mouse,rat, monkey and human. Is there any new application there? I will order the antibodies today. What's the best price for that?

ANSWER:

Thank you for contacting us.

I am very glad to be of service in helping you to find the correct product for your needs. Ab9332 is guaranteed by our Abpromise to work in WB on mouse tissues.We will be pleased to provide scientific support, replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

http://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.



Please do not hesitate to contact us if you need any more advice or information.

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Thanks for your information. It is glad to hear that it is Pan-specific phosphoserine antibody. I am going to use it to detect the phosphorylation of purified recombinant dynein intermediate chain of  mouse origin by a Ser/Thr kinase. Although I am not sure it's serine phosphorylation, it has higher possibility than Thr phosphorylation. Therefore, I am wondering if you could help me check if Abcam has  pan-specific phospho-Ser/Thr  antibody. Otherwise, I am going to get  this Pan-specific phosphoserine antibody and pan-specific phosphothreonine antibody(if there is any). if our research is qualified for discount, please let me know. Thanks

ANSWER:

We do offer two products which may meet your needs, ab17464 and ab117253.

While not truly Pan phospho Ser/ThrAb14764 detects phosphoserine or phosphothreonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. This product has been tested and guaranteed to work inICC/IF,ELISA,IP,and WB and has been referenced in seven publications to our knowledge. The product page is:

http://www.abcam.com/Phosphoserine-threonine-antibody-ab17464.html

Ab117253detects free and conjugated Phosphoserine/threonine as well as their modified cellular proteins. It does not cross-react with nonphosphorylated tyrosine, serine, threonine, AMP, or ATP. It has been tested and guaranteed to work inELISA,Dot Blot on human tissues. Again, I would like you to be aware that for each antibody of this type certain proteins known to contain phosphorylated serine and threonine may not be detected due to steric hindrance.The product page for this is:

http://www.abcam.com/Phosphoserine-threonine-antibody-ab117253.html

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: http://www.abcam.com/collaborationdiscount.

To determine if one of these products would qualify for participation in our 100% Abreview testing discount program could you tell me more about the application and species you would be testing the product on?

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

1. Dose this antibody recognize all kinds of phospho-serine sites or only recognize the phospho-serine site with certain amino acids around it? 2. May I buy and use a little amount of sample to test my protein first? If it works well, we will buy more. Thanks

ANSWER:

Thank you for contacting us.The phosphoserine antibody is pan-specific, however, steric hindrance by some adjacent amino acids may prevent efficient antibody recognition. We have not performed extensive studies to further characterize the affect of surrounding amino acids.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.


If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

http://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

I am trying to identify the kinase that phosphorylates a protein of my interest and would like to use your phosphoserine antibodies. But I am not sure if your antibody works regardless of the surrounding residues.

ANSWER:

Thank you for contacting Abcam regarding ab9332.


The phosphoserine antibody is pan-specific, however, steric hindrance by some adjacent amino acids may prevent efficient antibody recognition. We have not performed extensive studies to further characterize the affect of surrounding amino acids.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

We still have problem with the antibodies. We are now working on two antibodies which has beengenerouslysent from your company to replace the old vial we have purchased before. One is Anti-Phosphoserine( ab9332 ), the other is Anti-phosphoserien/threonine/tyrosine antibody (SPM101)(ab15556). Unfortunately,none of them could detect any bands for our IP samples. Quite often, the membranes are too dirty to see any bands.
By carefully going through the Western blotting of phospho-proteins protocol on Abcam website, we suspected two possible reasons that we might ignore before.
The first one is we load the samples on NuPAGE 10% Bis-Tris Gels(Novex life technologies) and transfer the membrane on iblot Gel transfer stacks nitrocellulose, regular (Invitrogen). It is different from what you recommend of using PVDF membrane. Do you think by using nitrocellulose membrane, the sensitivity of the membrane will be decreased?
The second one we did not filter the blocking media as you recommend before use. Could you explain a bit more about what to use and how to filter the 5%BSA?
All the bests!

ANSWER:

According to our records, ab9332 and ab15556was proving difficult to use inWB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.