Phosphoserine antibody (ab9332)

Product Name 

Phosphoserine antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Phosphoserine

Immunogen 

BSA and KLH-phosphoserine conjugates.

Specificity 

Recognize proteins phosphorylated on serine residues. Does not cross-reacted with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.5 µg/ml.
IP: Use at 10µg/0.25 mg of chromatography fractionated protein sample.
WB: Use at a concentration of 2 - 4 µg/ml.
To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse brain extract for Western Blotting.
Synthetic phosphopeptide or phosvitin for ELISA.

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: TBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for Phosphoserine antibody images, references, products related to ab9332 and other tools.

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References for Phosphoserine antibody (ab9332)

  This product has been used in: (two most recent references)

Wang P et al. POU homeodomain protein Oct-1 functions as a sensor for cyclic AMP. J Biol Chem 284:26456-65 (2009). WB. PubMed: 19617623

Ge C et al. Identification and Functional Characterization of ERK/MAPK Phosphorylation Sites in the Runx2 Transcription Factor. J Biol Chem 284:32533-43 (2009). WB. PubMed: 19801668

See all 8 publication references for this product.


If you publish research using ab9332 please let us know so that we can cite the reference on this datasheet.

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