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Product Name
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Phosphoserine antibody
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See all Phosphoserine antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Phosphoserine
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Immunogen
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BSA and KLH-phosphoserine conjugates.
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Specificity
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Recognize proteins phosphorylated on serine residues. Does not cross-reacted with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA.
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at a concentration of 0.5 µg/ml. IP: Use at 10µg/0.25 mg of chromatography fractionated protein sample. WB: Use at a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse brain extract for Western Blotting. Synthetic phosphopeptide or phosvitin for ELISA.
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Research areas
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Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation Signal Transduction >> Protein Phosphorylation >> pSer / pThr Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide Constituents: TBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Phosphoserine antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Phosphoserine antibody images, references, products related to ab9332 and other tools.
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Phosphoserine antibody images:
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Western blot
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies.
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
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References for Phosphoserine antibody (ab9332)
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This product has been used in: (two most recent references)
Wang P et al. POU homeodomain protein Oct-1 functions as a sensor for cyclic AMP. J Biol Chem 284:26456-65 (2009). WB. PubMed: 19617623
Ge C et al. Identification and Functional Characterization of ERK/MAPK Phosphorylation Sites in the Runx2 Transcription Factor. J Biol Chem 284:32533-43 (2009). WB. PubMed: 19801668
See all 8 publication references for this product.
If you publish research using ab9332 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Phosphoserine
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Phosphoserine antibody - more information
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Customer reviews (feedback) regarding Phosphoserine antibody |
Customer FAQs regarding Phosphoserine antibody |
Protocols for Phosphoserine antibody |
Price and availability of products related to Phosphoserine antibody |
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Phosphoserine antibody - related products:
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Phosphoserine antibody - other tools:
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Contact Abcam with a Technical Enquiry about Phosphoserine antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"