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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Phosphoserine antibody
See all Phosphoserine products (8) ...
Description
Rabbit polyclonal to Phosphoserine
Specificity
Recognize proteins phosphorylated on serine residues. Does not cross-reacted with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data.
Tested applications
ELISA, IP, WBmore details
Immunogen
BSA and KLH-phosphoserine conjugates.
Positive control
Mouse brain extract for Western Blotting. Synthetic phosphopeptide or phosvitin for ELISA.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.5 µg/ml
IP: Use at 10 µg/mg of lysate. (Use at 10µg/0.25 mg of chromatography fractionated protein sample.)
WB: Use a concentration of 2 - 4 µg/ml.(To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.)
Target
Relevance
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Alternative names
- phospho-Ser antibody
- pSER antibody
Anti-Phosphoserine antibody images:
Western blot
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
Western blot - Phosphoserine antibody (ab9332)
All lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)
Lane 1 : Whole cell lysate of monkey COS7 cells
Lane 2 : Whole cell lysate of monkey COS7 cells
Lysates/proteins at 25 µg per lane.
Secondary
An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution
Blocking Step: 10% Milk for 1 hour at room temperature
This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder
References for Anti-Phosphoserine antibody (ab9332)
This product has been referenced in:
- Karki Ret al. The MARCH family E3 ubiquitin ligase K5 alters monocyte metabolism and proliferation through receptor tyrosine kinase modulation. PLoS Pathog 7:e1001331 (2011).Read more (PubMed: 21490960) »
- Lu WTet al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB.Read more (PubMed: 21926477) »
See all 16 publications for this product
Publishing research using ab9332? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"