Products:Signal Transduction >> Metabolism >> Amino Acids
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ab17464 has been referenced in 8 publications.
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All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/1000 dilution
Lane 1 : Human THP-1 Whole Cell Extract
Lane 2 : Mouse Total Lung Extract
Lysates/proteins at 10 µg per lane.
Secondary
Alkaline phosphatase conjugated goat anti-rabbit antibody
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 5 minutes
This image is courtesy of an anonymous Abreview
To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.
All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/1000 dilution
Lane 1 : calyculin A treated A431 cells
Lane 2 : calyculin A treated A431 cells
All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/500 dilution
Lane 1 : Whole tissue lysate prepared from murine heart (control)
Lane 2 : Whole tissue lysate prepared from murine heart (control)
Lane 3 : Whole tissue lysate prepared from murine heart (PKC)
Lane 4 : Whole tissue lysate prepared from murine heart (PKC)
Lysates/proteins at 50 µg per lane.
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
The lower blot is to detect total mybpc3. It was probed with a non-abcam anti-mybpc3 antibody.
Image courtesy of an anonymous Abreview.
ab17464 staining Phosphoserine/threonine in murine cardiomyocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 1% Triton X-100, blocked with 10% horse serum for 1 hour at room temperature and then incubated with ab17464 at a 1/100 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
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