Phosphoserine/threonine antibody (ab17464)

Product Name 

Phosphoserine/threonine antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Phosphoserine/threonine

Immunogen 

Synthetic peptide.

Reacts with 
(species key)

N/A

Specificity 

Detects phosphoserine or phosphothreonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. The antibody does not recognize the nonphosphorylated form of these motifs, nor does it recognize other phosphoserine or phosphothreonine containing proteins and peptides.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions

ELISA: 1/1000.
IP: 1/100.
WB: 1/1000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications.
Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The Phospho-(Ser/Thr) Phe Antibody is a powerful tool for discovery of new
proteins containing this important regulatory motif.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

0.075 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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References for Phosphoserine/threonine antibody (ab17464)

  This product has been used in: (two most recent references)

Ye L et al. Bone morphogenetic protein-10 suppresses the growth and aggressiveness of prostate cancer cells through a Smad independent pathway. J Urol 181:2749-59 (2009). WB. PubMed: 19375725

Gao X et al. Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function. PLoS Pathog 5:e1000708 (2009). WB; Human. PubMed: 20041225

See all 4 publication references for this product.


If you publish research using ab17464 please let us know so that we can cite the reference on this datasheet.

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