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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Phosphoserine/threonine antibody
See all Phosphoserine/threonine products (2) ...
Description
Rabbit polyclonal to Phosphoserine/threonine
Specificity
Detects phosphoserine or phosphothreonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. The antibody does not recognize the nonphosphorylated form of these motifs, nor does it recognize other phosphoserine or phosphothreonine containing proteins and peptides.
Tested applications
ICC/IF, ELISA, IP, WBmore details
Immunogen
Synthetic peptide.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100
ELISA
ELISA: 1/1000ELISA: 1/1000
IP: 1/100
WB: 1/1000
Target
Relevance
A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The Phospho-(Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.
Alternative names
- Phosphoserine antibody
- Phosphothreonine antibody
Anti-Phosphoserine/threonine antibody images:
Western blot - Phosphoserine/threonine antibody (ab17464)
All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/1000 dilution
Lane 1 : Human THP-1 Whole Cell Extract
Lane 2 : Mouse Total Lung Extract
Lysates/proteins at 10 µg per lane.
Secondary
Alkaline phosphatase conjugated goat anti-rabbit antibody
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 5 minutes
This image is courtesy of an anonymous Abreview
Western blot - Phosphoserine/threonine antibody (ab17464)
To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.
Western blot - Phosphoserine/threonine antibody (ab17464)
All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/1000 dilution
Lane 1 : calyculin A treated A431 cells
Lane 2 : calyculin A treated A431 cells
Western blot - Phosphoserine/threonine antibody (ab17464)
All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/500 dilution
Lane 1 : Whole tissue lysate prepared from murine heart (control)
Lane 2 : Whole tissue lysate prepared from murine heart (control)
Lane 3 : Whole tissue lysate prepared from murine heart (PKC)
Lane 4 : Whole tissue lysate prepared from murine heart (PKC)
Lysates/proteins at 50 µg per lane.
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
The lower blot is to detect total mybpc3. It was probed with a non-abcam anti-mybpc3 antibody.
Image courtesy of an anonymous Abreview.
Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine/threonine antibody (ab17464)
ab17464 staining Phosphoserine/threonine in murine cardiomyocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 1% Triton X-100, blocked with 10% horse serum for 1 hour at room temperature and then incubated with ab17464 at a 1/100 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-Phosphoserine/threonine antibody (ab17464)
This product has been referenced in:
- Watanabe Set al. A novel glycosylation signal regulates transforming growth factor beta receptors as evidenced by endo-beta-galactosidase C expression in rodent cells. Glycobiology 21:482-92 (2011). Mouse.Read more (PubMed: 21062784) »
- Wentworth CCet al. Commensal-Epithelial Signaling Mediated via Formyl Peptide Receptors. Am J Pathol 177:2782-90 (2010). WB.Read more (PubMed: 21037077) »
See all 7 publications for this product
Publishing research using ab17464? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"