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Product name
Anti-Phosphotyrosine antibody [13F9]
See all Phosphotyrosine products (31) ...
Description
Mouse monoclonal [13F9] to Phosphotyrosine
Specificity
This monoclonal antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.
Tested applications
ELISA, WBmore details
Immunogen
Phosphotyrosine conjugated KLH
General notes
This product contains 0.01% (w/v) Sodium Azide preservative.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2
Concentration
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Purity
Ascites
Clonality
Monoclonal
Clone number
13F9
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
This product was assayed against phosphotyrosine conjugated BSA by ELISA using HRP Goat-anti-Mouse IgG for detection.
A working dilution of 1:2,000 to 1:10,000 is suggested for this assay.
For immunoblotting, dilute the antibody 1:1000 immediately before use.
Researchers should determine optimal titers for other applications.
Target
Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
References for Anti-Phosphotyrosine antibody [13F9] (ab2287)
ab2287 has not yet been referenced specifically in any publications.
Publishing research using ab2287? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"