Phosphotyrosine antibody (HRP) (ab9329)

Product Name 

Phosphotyrosine antibody (HRP)

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Phosphotyrosine (HRP)

Immunogen 

Chemical / Small Molecule conjugated to KLH.

Specificity 

Recognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at 2:1000
Western blot: use at 8:1000
IP: use at 4µg/250 mg protein sample of treated cell
Not recommended for immunoblotting of trace cellular phosphoprotein.
Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.

Positive control 
(see definition)

Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.

Research areas 

Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Phosphatases

Relevance 

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Conjugation notes 

HRP (Horse Radish Peroxidase)

Storage buffer 

Phosphate Buffered Saline

Purification notes 

Immunoaffinity chromatography with phosphotyrosine-agarose.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

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References for Phosphotyrosine antibody (HRP) (ab9329)

  This product has been used in:

McLaughlin NJ et al. Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180:8192-203 (2008). WB. PubMed: 18523285

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