Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Phosphatases
Overview
Product name
Anti-Phosphotyrosine antibody (HRP)
See all Phosphotyrosine products (31) ...
Description
Rabbit polyclonal to Phosphotyrosine (HRP)
Conjugation
HRP
Conjugation notes
HRP (Horse Radish Peroxidase)
Specificity
Recognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.
Tested applications
WB, IP, ELISAmore details
Immunogen
Chemical / Small Molecule conjugated to KLH.
Positive control
Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Phosphate Buffered Saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Immunoaffinity chromatography with phosphotyrosine-agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Phosphatases
-
Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Target
Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Anti-Phosphotyrosine antibody (HRP) images:
References for Anti-Phosphotyrosine antibody (HRP) (ab9329)
This product has been referenced in:
- McLaughlin NJet al. Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180:8192-203 (2008). WB.Read more (PubMed: 18523285) »
See 1 publication for this product
Publishing research using ab9329? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"