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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Phosphatases
Overview
Product name
Anti-Phosphotyrosine antibody
See all Phosphotyrosine products (31) ...
Description
Rabbit polyclonal to Phosphotyrosine
Specificity
Recognize proteins and peptides phosphorylated on tyrosine residues. Not cross-reacted with phosphoserine and threonine.
Tested applications
ELISA, IP, WB, ICC/IF, IHC-P, IHC-Frmore details
Immunogen
Chemical / Small Molecule conjugated to KLH.
Positive control
Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.1M Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IP: Use at an assay dependent dilution. (10 µg/500 µg protein sample.)
WB: 1/250
ICC/IF: Use at an assay dependent dilution. (PubMed: 21084607)
IHC-P
IHC-P: 1/50IHC-P: 1/50
IHC-Fr
IHC-Fr: 1/50IHC-Fr: 1/50
Target
Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
References for Anti-Phosphotyrosine antibody (ab9319)
This product has been referenced in:
- Zhong Zet al. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region. J Neurosci 30:15521-34 (2010). ICC/IF, IP.Read more (PubMed: 21084607) »
- Moya Aet al. Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization. J Biol Chem 283:25475-84 (2008). WB.Read more (PubMed: 18617510) »
See all 2 publications for this product
Publishing research using ab9319? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"