Anti-Piccolo antibody [6H9-B6] (ab101654)

Overview

  • Product nameAnti-Piccolo antibody [6H9-B6]See all Piccolo primary antibodies ...
  • Description
    Mouse monoclonal [6H9-B6] to Piccolo
  • SpecificityNo cross reactivity to Bassoon.
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human Piccolo (BC001304.1).

  • Positive control
    • Rat brain tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number6H9-B6
  • IsotypeIgG2b
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab101654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 567 kDa. 1 µg/mL of ab101654 was sufficient for detection of Piccolo in 20 µg of lysate by colorimetric immunoblot analysis using goat antimouse IgG:HRP as the secondary antibody.
ELISA Use at an assay dependent dilution.

Target

  • FunctionMay act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
  • Sequence similaritiesContains 2 C2 domains.
    Contains 1 PDZ (DHR) domain.
  • DomainC2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
  • Cellular localizationCell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
  • Target information above from: UniProt accession Q9Y6V0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ACZ antibody
    • Aczonin antibody
    • Brain-derived HLMN protein antibody
    • DKFZp779G1236 antibody
    • KIAA0559 antibody
    • Multidomain presynaptic cytomatrix protein antibody
    • PCLO antibody
    • PCLO_HUMAN antibody
    • Piccolo (presynaptic cytomatrix protein) antibody
    • Piccolo, mouse, homolog of antibody
    • Pico antibody
    • Presynaptic cytomatrix protein antibody
    • Protein piccolo antibody
    see all

Anti-Piccolo antibody [6H9-B6] images



  • Predicted band size : 567 kDa
    Western blot detection of Picalolo in (1) Mouse brain lysate and (2) Rat brain lysate by ab101654.

References for Anti-Piccolo antibody [6H9-B6] (ab101654)

ab101654 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Yes, ab101654 is the only mous monoclonal antibody we have for Piccolo. It has not yet been tested with mouse samples and in IHC-P (paraffin-embedded PFA-fixed tissue sections). We cannot guarantee if and how well t...

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Vielen Dank für Ihren Anruf. Wie versprochen hatte ich für den anti-Piccolo- Antikörper ab101654 nachgefragt, ob das Epitop kartiert worden ist. Daraufhin hat man mir die Immunogensequenz geschickt, die zur Generierung dieses Antikörpers verwendet wurd...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"