Overview

  • Product name
    Anti-Piccolo antibody [6H9-B6]
    See all Piccolo primary antibodies
  • Description
    Mouse monoclonal [6H9-B6] to Piccolo
  • Specificity
    No cross reactivity to Bassoon.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human Piccolo (BC001304.1).

  • Positive control
    • Rat brain tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    6H9-B6
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 567 kDa. 1 µg/mL of ab101654 was sufficient for detection of Piccolo in 20 µg of lysate by colorimetric immunoblot analysis using goat antimouse IgG:HRP as the secondary antibody.
ELISA Use at an assay dependent dilution.

Target

  • Function
    May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
  • Sequence similarities
    Contains 2 C2 domains.
    Contains 1 PDZ (DHR) domain.
  • Domain
    C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
  • Cellular localization
    Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACZ antibody
    • Aczonin antibody
    • Brain-derived HLMN protein antibody
    • DKFZp779G1236 antibody
    • KIAA0559 antibody
    • Multidomain presynaptic cytomatrix protein antibody
    • PCLO antibody
    • PCLO_HUMAN antibody
    • Piccolo (presynaptic cytomatrix protein) antibody
    • Piccolo, mouse, homolog of antibody
    • Pico antibody
    • Presynaptic cytomatrix protein antibody
    • Protein piccolo antibody
    see all

Anti-Piccolo antibody [6H9-B6] images



  • Predicted band size : 567 kDa
    Western blot detection of Picalolo in (1) Mouse brain lysate and (2) Rat brain lysate by ab101654.

References for Anti-Piccolo antibody [6H9-B6] (ab101654)

This product has been referenced in:
  • Mateos-Hernández L  et al. Quantitative proteomics reveals Piccolo as a candidate serological correlate of recovery from Guillain-Barré syndrome. Oncotarget 7:74582-74591 (2016). ELISA ; Human . Read more (PubMed: 27776345) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

Yes, ab101654 is the only mous monoclonal antibody we have for Piccolo. It has not yet been tested with mouse samples and in IHC-P (paraffin-embedded PFA-fixed tissue sections). We cannot guarantee if and how well t...

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Vielen Dank für Ihren Anruf. Wie versprochen hatte ich für den anti-Piccolo- Antikörper ab101654 nachgefragt, ob das Epitop kartiert worden ist. Daraufhin hat man mir die Immunogensequenz geschickt, die zur Generierung dieses Antikörpers verwendet wurd...

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