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All lanes : Anti-Piccolo antibody - Synaptic Marker (ab20664) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate with
Lane 4 : Brain (Mouse) Tissue Lysate with
Lysates/proteins at 30 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 520 kDa
Observed band size : 275 kDa (why is the actual band size different from the predicted?)
Additional bands at : 31 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
0
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