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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Overview
Product name
Piccolo peptide
See all Piccolo products (2) ...
Protein description
Synthetic peptide derived from within residues 600 - 700 of Rat Piccolo.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab20664 - Piccolo antibody - Synaptic Marker.
Protein length
5085 amino acids
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
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Western blot - Piccolo antibody - Synaptic Marker (ab20664)
(enlarge)
Applications
Protein info
Function
May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
Sequence similarities
Contains 2 C2 domains.
Contains 1 PDZ (DHR) domain.
Domain
C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
Cellular localization
Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
Target information above from: UniProt accessionQ9Y6V0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Piccolo peptide images:
Western blot - Piccolo antibody - Synaptic Marker (ab20664)
All lanes : Anti-Piccolo antibody - Synaptic Marker (ab20664) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate with Piccolo peptide (ab23400) at 1 µg/ml
Lane 4 : Brain (Mouse) Tissue Lysate with Piccolo peptide (ab23400) at 1 µg/ml
Lysates/proteins at 30 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 520 kDa
Observed band size : 275 kDa (why is the actual band size different from the predicted?)
Additional bands at : 31 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
References for Piccolo peptide (ab23400)
ab23400 has not yet been referenced specifically in any publications.
Publishing research using ab23400? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"