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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Lipid Kinases
Overview
Product name
Pik3ca + PIK3R1 protein (Active)
Protein description
Recombinant full-length Mouse Pik3ca (amino acids 1-1068) and full-length Human PIK3R1 (amino acids 1-724) were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins, MWt 111 and 86kDa respectively. (Swiss-Prot P42337 and P27986).
Properties
Purification notes
Purity was determined to be >90% by densitometry.
Biological activity
The Specific activity of ab85833 was determined to be 5147 RLU/min/mg.
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 150mM Imidazole
Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0
Concentration
Concentration information loading...
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Lipid Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Relevance
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach.
Pik3ca + PIK3R1 protein (Active) images:
Functional Studies - Pik3ca + PIK3R1 protein (Active) (ab85833)
The Specific activity of ab85833 was determined to be 5147 RLU/min/mg.
SDS-PAGE - Pik3ca + PIK3R1 protein (Active) (ab85833)
SDS-PAGE showing ab85833 with bands at approximately 111 (Pik3ca) and 86kDa (PIK3R1).
References for Pik3ca + PIK3R1 protein (Active) (ab85833)
ab85833 has not yet been referenced specifically in any publications.
Publishing research using ab85833? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"