Pik3ca + PIK3R1 protein (Active) (ab85833)

Product Name 

Pik3ca + PIK3R1 protein (Active)

Product type 

Proteins and Peptides

Peptide 

Recombinant full-length Mouse Pik3ca (amino acids 1-1068) and full-length Human PIK3R1 (amino acids 1-724) were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins, MWt 111 and 86kDa respectively. (Swiss-Prot P42337 and P27986).

Specificity 

The Specific activity of ab85833 was determined to be 5147 RLU/min/mg.

Tested applications 
(see key)

FuncS, SDS-PAGE, WB

Application notes 
(see key)

Recommended dilutions
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Lipid Kinases

Relevance 

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach.

Storage buffer 

Preservative: 150mM Imidazole
Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Phosphate MSDS

Purification notes 

Purity was determined to be >90% by densitometry.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

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