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Pindolol Fluorescent ligand (Red) (ab118170)

Q&A (5)

Overview

  • Product namePindolol Fluorescent ligand (Red)
  • Description

    Fluorescent β2 antagonist

  • Alternative names
    ß3-633-AN4
  • Biological descriptionFluorescent β2 antagonist (pKD values are 7.01, 7.96 and 6.42 at β1, β2 and β3 respectively). Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics. Intrinsic, weak partial agonist activity at β1, β2 and β3 receptors (SEAP reporter gene assay).

    Excitation wavelength: 633 nm

    Emission wavelength: 650 nm

  • Purity> 97%

    • Properties

  • Selectivityβ1:7.01, β2:7.96, β3:6.42
  • Excitation633nm
  • Emission650nm
  • Molecular Weight833.00
  • Molecular formulaC46H53BF2N6O7S
  • Validation notesThe affinity of ab118170 at β1, β2 and β3 adrenoceptors was determined in a whole cell radioligand binding assay using [3H]-CGP12177.
    ab118170 was shown to antagonize the activity of the non-selective β agonist, isoprenaline, in three recombinant CHO cell lines expressing either the Human β1, β2 or β3 receptor and a cyclic AMP-responsive secreted placental alkaline phosphatase (SPAP) reporter gene.
    Addition of ab118170 to the basal or forskolin-stimulated cells did not significantly alter basal and stimulated SPAP levels.
    To determine the KD for ab118170 at β1, β2 and β3 receptors, cells were treated with isoprenaline alone, or in the presence of ab118170, and the cyclic AMP-induced expression of SPAP measured.
    The KD was calculated from the rightward shift of the agonist response curve.
  • Storage instructionsStore at -20°C. Avoid exposure to light.
  • HandlingFor imaging at β1 / β2 / β3 adrenoceptors use solutions up to 100 nM in DMSO.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab118170 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Small Molecule Fluorescent Optical Detection Small Molecule Fluorescent Optical Detection: Use at an assay dependent concentration.
    Receptor Localization Receptor Localization: Use at an assay dependent concentration.
    Fluorescent Cell Imaging Fluorescent Cell Imaging: Use at an assay dependent concentration.

    Pindolol Fluorescent ligand (Red) images

    • Fluorescent Cell Imaging - Pindolol Fluorescent ligand (Red) (ab118170)
      Fluorescent Cell Imaging - Pindolol Fluorescent ligand (Red) (ab118170)
      ab118170 ligand binding and displacement.
      Top (a, C): ab118170 binding to two different live cell lines expressing β1 (A) or β2 (C) adrenoceptors.
      Bottom (B, D): Binding blocked in the same two cell lines β1 (B) or β2 (D) by the unlabelled competitor ICI118551 (1 µM).
      Nuclei have been counter-stained with Hoechst.

    References for Pindolol Fluorescent ligand (Red) (ab118170)

    ab118170 has not yet been referenced specifically in any publications.

    Publishing research using ab118170 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab118170

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    I wonder if we can purchase Bodipy fluorophore which is used to label the product b118165 from you.
    We have observed non-specific binding of b118165 on artificial liposomes and we would like to find out if the dye itself binds to liposomes non-speci...

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    Thank you for contacting us.

    We unfortunately do not sell Bodipy dyes. These however can be easily available form XXXX


    Please click the link below to know which Bodipy dye is attached to products;

    http://www.abcam.com/index...

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    Inquiry: Hi, Can you tell me if any of your fluorescent beta2 adrenergic receptor ligands (ab118171 (S)-Carazolol Fluorescent ligand (Red) ab118169 (S)-Propranolol Fluorescent ligand (Red) ab118167 Alprenolol Fluorescent ligand (Red) ab118170 Pindolol Flu...

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    Thank you for contacting us and for your interest in our products.

    I am sorry to confirm that as far as we are aware,the fluorescent ligandshave not been tested in flow cytometry. All tested and guaranteed applications are specified on our data...

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    We are interested in fluorescently labeled 'beta2-adrenergic receptor' ligands. Particularly in the following products:
    http://www.abcam.com/S-Propranolol-Fluorescent-ligand-Red-ab118169.html,
    http://www.abcam.com/Alprenolol-Fluorescent-ligand-Red...

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    I have received further information from lab about the solubility.

    They said, there are no known problems of using these compounds in aqueous buffer, however no specific numeric data exists. These fluorescent ligands can be used in other organic ...

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    We are interested in fluorescently labeled 'beta2-adrenergic receptor' ligands. Particularly in the following products:
    http://www.abcam.com/S-Propranolol-Fluorescent-ligand-Red-ab118169.html,
    http://www.abcam.com/Alprenolol-Fluorescent-ligand-Red...

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    Thank you for contacting us.

    The product can be dissolved to 10 mM or 1 mM (to give a larger volume) in DMSO.

    · Appropriate final DMSO concentration is 0.1% (in buffer)

    · We are not aware of aqueous solubility of t...

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    I'm very interested, but I need to know the Kd-values of the bodipy ligands listed below:

    Thank you for contacting Abcam.

    I have included a pdf file which contains the pKd values for the products as you have asked. This information and more is also available
    on the individual product pages on the abcam website (e.g. http://www.ab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"