Anti-Piwi antibody (ab5207)

I am writing to hear whether it is possible to buy small aliquots of the following antibodies or whether it would be possible to ask for free aliquots to see if the antibodies work in our model organism, the planarian:

*Piwi antibody (ab5207) *Argonaute 4 antibody (ab3600) *Nanos homologue 2 (NANOS2) antibody (ab15731) *Nanos Homologue 1 (NANOS1) antibody (ab15733)

I am a PhD student working on a characterization of the planarian stem cells (the neoblasts) and of germline stem cells. The planarian that we are using in the lab is the flatworm Schmidtea mediterranea (phylum Platyhelminthes, class Turbellaria, order Seriata, suborder Tricladida). I have studied the expression pattern of nanos- and piwi-like genes by whole mount in situ hybridization, but would like to try immunostaining with the corresponding antibodies to get a clearer pattern. Since we do not know whether the antibodies that you offer will work on the planarian, we would like to buy/to ask for a small aliquot to try it out before buying the entire sample. If the antibody works, we will buy the entire sample.

Other antibodies that we are interested in trying for the characterization of the planarian stem cells and germline stem cells are the following:

* Sox2 antibody (ab15830) * Oct4 antibody (ab18976) * Fragilis antibody (ab15592) * Nanog (ab21603) * SCF (ab17521) * VASA (ab13840) * Beclin 1 antibody (ab16998)

I am looking forward to hearing from you!

ANSWER:

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement.

Should you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image).