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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Piwi antibody
Description
Rabbit polyclonal to Piwi
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide derived from residues 350 - 450 of Drosophila melanogaster Piwi.
.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> RNAi >> RISC
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
WB: 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Piwi peptide (ab14803).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Piwi mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. It is expressed somatically in the ovariole terminal filament and epithelial sheath cells. Expressed in the germarium at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10 and eventually accumulating in early embryos.
Cellular localization
Nuclear
Alternative names
- HIWI antibody
- P-element induced wimpy testis antibody
- Piwi antibody
see all
Database links
- Entrez Gene: 34521 Fruit fly (Drosophila melanogaster)
- SwissProt: Q9VKM1 Fruit fly (Drosophila melanogaster)
Anti-Piwi antibody images:
Western blot - Piwi antibody (ab5207)
Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 97 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 53 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-Piwi antibody (ab5207)
Predicted band size : 97 kDa
Drosophila embryo lysate, 8% TRIS SDS-PAGE.
The arrow indicates Piwi at a predicted MW of 90 kDa.
Pre-immune sera was used as a control and showed no equivalent band (data not shown).
Ahmet Denli
Ahmet Denli
References for Anti-Piwi antibody (ab5207)
This product has been referenced in:
- Moshkovich Net al. RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. Genes Dev 25:1686-701 (2011). WB; Fruit fly (Drosophila melanogaster).Read more (PubMed: 21852534) »
- Klenov MSet al. Separation of stem cell maintenance and transposon silencing functions of Piwi protein. Proc Natl Acad Sci U S A 108:18760-5 (2011).Read more (PubMed: 22065765) »
See all 4 publications for this product
Publishing research using ab5207? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"