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Product Name
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Piwi antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Piwi
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Immunogen
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Synthetic peptide derived from residues 350 - 450 of Drosophila melanogaster Piwi.
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Reacts with
(species key)
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Dm
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Piwi peptide (ab14803).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> RNAi >> RISC
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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Piwi mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. It is expressed somatically in the ovariole terminal filament and epithelial sheath cells. Expressed in the germarium at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10 and eventually accumulating in early embryos.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 34521 (Fruit fly (Drosophila melanogaster))
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| SwissProt | | Q9VKM1 (Fruit fly (Drosophila melanogaster))
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Piwi antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Piwi antibody images, references, products related to ab5207 and other tools.
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Piwi antibody images:
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 - Piwi antibody (ab5207)
Predicted band size : 97 kDa
Drosophila embryo lysate, 8% TRIS SDS-PAGE.
The arrow indicates Piwi at a predicted MW of 90 kDa.
Pre-immune sera was used as a control and showed no equivalent band (data not shown).
Ahmet Denli
Ahmet Denli
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References for Piwi antibody (ab5207)
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General references:
Parker JS, Roe SM, Barford D. Structural insights into mRNA recognition from a PIWI domain-siRNA guide complex. Nature. 2005 Mar 31;434(7033):663-6.
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Search PubMed (MEDLINE) for references to Piwi
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Piwi antibody - more information
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Customer reviews (feedback) regarding Piwi antibody |
Customer FAQs regarding Piwi antibody |
Protocols for Piwi antibody |
Price and availability of products related to Piwi antibody |
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Piwi antibody - related products:
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Piwi antibody - other tools:
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Contact Abcam with a Technical Enquiry about Piwi antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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