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Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)

Q&A (13)Specific References (2)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates (42 week aged placenta) using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab29745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)

This product has been referenced in:
  • Larsen MH  et al. Human leukocyte antigen-G in the male reproductive system and in seminal plasma. Mol Hum Reprod 17:727-38 (2011). WB . Read more (PubMed: 21813635) »
  • Weitzel LR  et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat 124:297-305 (2010). WB . Read more (PubMed: 20087651) »

See all 2 Publications for this product

Publishing research using ab29745 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit a Question for ab29745

Displaying 1 - 10 of 13 results for Q&A

10
Votes: Highest First

We purchased Placenta tissue lysate on August 31 2012, which is supposed to be 100ug, equaling 26,6 ul. However the vial had fluid between the cap and thread and held only about 15ul. Please send us a replacement.

Thank you for contacting us.

I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial.

Please be sure to spin the via...

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Thank you for your reply. I have provided answers to all you questions, noted in red, below.

What is the main objective of these studies? The objective of these studies is to identify BCRP/ABCG2 in 4 different cell lines in order tocooberatewit...

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Thank you for getting back to me promptly.

It is important to note that the actual (apparent) molecular weight values for the prestained markers can be different from the non-stained markers and also in different gel types.

When a prot...

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Thank you for the second vial of my replacement positive control (ab29743).



I have been using the second vial of the positive control provided in my recent experiments (5 and 10 µg total protein per lane after denaturing for 5...

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Thank you for getting back to me and for sharing your results with me.

The storage buffer of the positive control lysate contains beta mercaptoethanol (reducing agent) so it is very unlikely that the protein is dimerized particularly if the WB w...

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Thank you for your email indicating that you have placed an order for a replacement vial of ab29743. I was wondering what the status of this order was? I am aware that it is coming up to the weekend and am concerned that it is going to be left unrefrigera...

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Thank you for your e-mail.

I can confirm that the package has been delivered this morning 20Jul12 at 10:14 GMT and the person who signed for was: J. XXX.

Please check your Goods In Department. For your reference, tracking number was: 0...

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Thank you for your advise regarding ab29743.

As you had mentioned previously, I am due two replacement positive control vials, but hadonly accepted one to check that it was working under my western blot conditions. As you are confident that I a...

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Thank you for your new contact.

As we discussed and agreed previously, I have placed the second free of charge replacement vial (ab29743) - exchange for the original product ab29745. The new order number is No: 1125597.

Currently, we d...

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I would like to sincerely thank you for all your help and advice. I shall keep in touch and let you know the progress of the replacement positive control.

Do you know whenab29743 will be dispatched?

Thank you again.

Best W...

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Just to confirm that you do not have to pay for the free of charge replacement vial. I have raised a credit note for the incorrect amount of £237.60 that has been charged. The reference number for the credit note is: CN21732

Apologies! Read More

Thank you again for all your help concerningab29743 and providing me with a replacement positive control inab29743 [CCE3860585].

I have triedab29743 (10 and 20 µg total protein per lane after denaturing for 5 minutes at 95C, as stated in ...

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Thank you for sharing your recent results and providing the WB images..

My view is that you detect BCRP/ABCG2 in the positive control as well as in the samples.

However, there are a few things to consider:

There are some spli...

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I would like to sincerely thank you for all your help and advice. I shall keep in touch and let you know the progress of the replacement positive control.

Do you know whenab29743 will be dispatched?



Thank you again.

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Thank you for your e-mail.

Currently, the vial is in transit and we are waiting to get it from our Boston office. Hopefully today or Tuesday we will be able to dispatch it for you.

If you need any further assistance in the future, please...

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I would like to thank you for all your help regarding my enquiry concerningab29745 [CCE3860585]



I'm glad that you are happy with my protocol. I was wondering if you knew the optimalconcentration range recommended for use of a positive c...

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Thank you for your response.

I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful. Normally, we would suggest loading 20-25 ug total protein per lane.

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Thank you for your response regarding my enquiry concerningab29745 [CCE3860585]



Yes, I was also under the impression thatab29745 would be a reliable positive control for use in western blotting.



I shall investigateab2...

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Thank you for getting back to me promptly and for providing some further details.

The protocol seems to be absolutely fine. Since you have applied a sensitive LICOR Odyssey IR Imaging System for detection, the concentrations of the primary and th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"