![Western blot - Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289) image](#glyco1.png)
![Immunohistochemistry (Paraffin-embedded sections) - Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289) image](#glyco2.png)
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Products:Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Overview
Product name
Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y]
See all Placental Protein 14 / Glycodelin A products (6) ...
Description
Rabbit monoclonal [EP870Y] to Placental Protein 14 / Glycodelin A
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues near the C-terminal of human PAEP/PEG.
Positive control
Placenta tissue K562 treated with TPA
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP870Y
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250 - 1/500.Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P: 1/100 - 1/250.
Application notes
Is unsuitable for ,Flow Cyt or IP.
Target
Function
This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family.
Post-translational
modifications
At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction.
Cellular localization
Secreted.
Target information above from: UniProt accessionP09466
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- alpha uterine protein antibody
- GD antibody
- GdA antibody
see all
Database links
- Entrez Gene: 5047 Human
- Omim: 173310 Human
- SwissProt: P09466 Human
- Unigene: 532325 Human
Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y] images:
Western blot - Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289)
![Western blot - Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289)](/ps/datasheet/Images/53/ab53289/glyco1.png)
All lanes : Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289) at 1/500 dilution
Lane 1 : K562
Lane 2 : K562 + TPA treatment
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 21 kDa
Observed band size : 21 kDa
Immunohistochemistry (Paraffin-embedded sections) - Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289)
![Immunohistochemistry (Paraffin-embedded sections) - Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289)](/ps/datasheet/Images/53/ab53289/glyco2.png)
Placenta stained with ab53289 at 1/100 - 1/250 dilution
References for Anti-Placental Protein 14 / Glycodelin A antibody [EP870Y] (ab53289)
ab53289 has not yet been referenced specifically in any publications.
Publishing research using ab53289? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"