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RabMAbs

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Submit an Abreview Q&A (2)Specific References (4)

Overview

  • Product nameAnti-Placental alkaline phosphatase (PLAP) antibody [SP15]See all Placental alkaline phosphatase (PLAP) primary antibodies ...
  • Description
    Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
  • Tested applicationsIHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein (Human).

  • EpitopeNot determined
  • Positive controlSeminoma and placenta
  • General notes


    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Tissue culture supernatant
  • PurityTissue culture supernatant
  • Primary antibody notes This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
  • Clonality Monoclonal
  • Clone numberSP15
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab16695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
WB
  • Application notesIHC-P: 1/100. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    WB: Use at an assay dependent dilution PMID 16424182.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Alternative names
        Alkaline phosphatase antibodyAlkaline phosphatase placental antibodyAlkaline phosphatase placental type antibody
        Alkaline phosphatase Regan isozyme antibodyALP antibodyAlp1 antibodyALPP antibodyFLJ61142 antibodyGerm-cell alkaline phosphatase antibodyGlycerophosphatase antibodyGlycerophosphatase antibodynagao isozyme antibodyOTTHUMP00000164354 antibodyPALP antibodyPlacental alkaline phosphatase 1 antibodyplacental heat-stable alkaline phosphatase antibodyplacental type antibodyPLAP 1 antibodyPLAP antibodyPLAP-1 antibodyPLAP1 antibodyPPB1_HUMAN antibodyRegan isozyme antibody
      see all

    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
      Human seminoma stained with ab16695.

    References for Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    This product has been referenced in:
    • Saffarini CM  et al. Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice. J Androl 33:505-13 (2012). IHC-P ; Mouse . Read more (PubMed: 21868749) »
    • Schmid F  et al. Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Degradation by Alkaline Phosphatase. J Biol Chem 287:32525-34 (2012). WB . Read more (PubMed: 22851169) »

    See all 4 Publications for this product

    Publishing research using ab16695 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab16695 Submit a Question for ab16695

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    We are interested in your products: “ab16695”, “ab16517” and “ab70988” but we need to know more about the sequence targeted by it. We would like to have as much information as possible about this question.

    Thank you for your enquiry.

    Here are the answers to your questions:

    1. ab16517 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
    2. ab16695 - immunogen is Full length native protein (puri...

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    Is there a western blot image available for this antibody?

    Please see the western blot in following reference noted under the 'References' section of the online datasheet for ab16695: Taylor DD et al. Pregnancy-associated exosomes and their modulation of T cell signaling. J Immunol 176:1534-42 (2006). WB; Hum...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"