RabMAb

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Overview

  • Product nameAnti-Placental alkaline phosphatase (PLAP) antibody [SP15]See all Placental alkaline phosphatase (PLAP) primary antibodies ...
  • Description
    Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein (Human).

  • EpitopeNot determined
  • Positive control
    • Seminoma and placenta
  • General notes


    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Tissue culture supernatant
  • PurityTissue culture supernatant
  • Primary antibody notes This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
  • Clonality Monoclonal
  • Clone numberSP15
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab16695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesIHC-P: 1/100. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    WB: Use at an assay dependent dilution PMID 16424182.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesBelongs to the alkaline phosphatase family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alkaline phosphatase antibody
      • Alkaline phosphatase placental antibody
      • Alkaline phosphatase placental type antibody
      • Alkaline phosphatase Regan isozyme antibody
      • ALP antibody
      • Alp1 antibody
      • ALPP antibody
      • FLJ61142 antibody
      • Germ-cell alkaline phosphatase antibody
      • Glycerophosphatase antibody
      • Glycerophosphatase antibody
      • nagao isozyme antibody
      • OTTHUMP00000164354 antibody
      • PALP antibody
      • Placental alkaline phosphatase 1 antibody
      • placental heat-stable alkaline phosphatase antibody
      • placental type antibody
      • PLAP 1 antibody
      • PLAP antibody
      • PLAP-1 antibody
      • PLAP1 antibody
      • PPB1_HUMAN antibody
      • Regan isozyme antibody
      see all

    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] images

    • Human seminoma stained with ab16695.
    • Immunohistochemical analysis of paraffin-embedded Human seminoma tissue labelling Alkaline Phosphatase with ab137879 at 1/50 dilution.

    References for Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    This product has been referenced in:
    • Saffarini CM  et al. Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice. J Androl 33:505-13 (2012). IHC-P ; Mouse . Read more (PubMed: 21868749) »
    • Schmid F  et al. Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Degradation by Alkaline Phosphatase. J Biol Chem 287:32525-34 (2012). WB . Read more (PubMed: 22851169) »

    See all 4 Publications for this product

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    Here are the answers to your questions:

    1. ab16517 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
    2. ab16695 - immunogen is Full length native protein (p...

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    Please see the western blot in following reference noted under the 'References' section of the online datasheet for ab16695: Taylor DD et al. Pregnancy-associated exosomes and their modulation of T cell signaling. J Immunol 176:1534-42 (2006). WB; ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"