Overview
- Product nameAnti-Placental lactogen antibodySee all Placental lactogen primary antibodies ...
- DescriptionRabbit polyclonal to Placental lactogen
- Tested applicationsICC/IF, WB, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Purified human placental lactogen
- Positive controlPlacenta
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: Sodium Azide
Constituents: 10mM PBS, BSA. pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab15554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. |
| IHC-P | IHC-P: 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
- FunctionProduced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization.
- Sequence similaritiesBelongs to the somatotropin/prolactin family.
- Cellular localizationSecreted.
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Database links
- Entrez Gene: 1442 Human
- Entrez Gene: 1443 Human
- Omim: 150200 Human
- SwissProt: P01243 Human
- Unigene: 654390 Human
- Unigene: 654515 Human
Target information above from: UniProt accession
P01243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Choriomammotropin antibodyChorionic somatomammotropin A antibodyChorionic somatomammotropin hormone 1 antibody
- Chorionic somatomammotropin hormone antibodyCS1 antibodyCSA antibodyCSH_HUMAN antibodyCSH1 antibodyCSH1 antibodyCSH2 antibodyCSMT antibodyhCSA antibodyLactogen antibodyPL antibodyPL1 antibodyPlacental lactogen antibodySomatotropin precursor antibody
see all
Anti-Placental lactogen antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Placental lactogen antibody (ab15554)ab15554 staining Human placenta by Immunohistochemistry (FFPE-sections).
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Western blot - Placental lactogen antibody (ab15554)Anti-Placental lactogen antibody (ab15554) at 1/100 dilution + Human recombinant hPL at 0.1 µg
Secondary
HRP conjugated donkey anti-rabbit IgG
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 25 kDa
Additional bands at : 10 kDa,14 kDa,45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutesThis image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
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Immunocytochemistry/ Immunofluorescence - Human placental lactogen antibody (ab15554)This image is courtesy of an Abreview submitted by Dr Stoil Dmitrovab15554 at 1/200 staining human BeWo (placenta choriocarcinoma) cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A rhodamine conjugated goat anti-rabbit antibody was used as the secondary. The left hand panel of the image shows DAPI counterstain. -
Western blot - Placental lactogen antibody (ab15554)Anti-Placental lactogen antibody (ab15554) at 1/25 dilution + Human placenta lysate
Predicted band size : 25 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
References for Anti-Placental lactogen antibody (ab15554)
This product has been referenced in:
- Drukker M et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). ICC/IF ; Human . Read more (PubMed: 22634564) »
- Adachi K et al. Role of SOX2 in maintaining pluripotency of human embryonic stem cells. Genes Cells : (2010). Read more (PubMed: 20384793) »
