Plasma Kallikrein 1B antibody - Apple Domain 2 (ab43084)

Product Name 

Plasma Kallikrein 1B antibody - Apple Domain 2

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Plasma Kallikrein 1B - Apple Domain 2

Immunogen 

(Peptide available as ab43117.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab43084 recognizes the zymogen form of plasma kallikrein and the heavy chain of factor XIIa cleaved plasma kallikrein, but not the kallikrein heavy chain, and can be used to distinguish the two forms.

We have a range of domain specific antibodies for this target. For a full list please see all Plasma Kallikrein 1B antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1:1000. Predicted molecular weight: 71 kDa. A 1:5000 dilution is recommended for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Glycosylation and other post translational modifications give plasma kallikrein a higher apparent molecular weights of 88 and 85 kDa Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins

Relevance 

Plasma Kallikrein 1B is a serine proteinase of the S1 family It is secreted by the liver, kidney, adrenal gland and placenta. The zymogen is referred to as prekallikrein (PK) and is activated by Factor XIIa to an active two chain form, plasma kallikrein. In Fletcher syndrome plasma kallikrein is deficient, resulting in a prolonged partial thromboplastin time (PT).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.5% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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