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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Kallikrein 1B antibody (ab44392)
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Product Name
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Plasma Kallikrein 1B antibody
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See all Plasma Kallikrein 1B antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Plasma Kallikrein 1B
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Immunogen
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A synthetic peptide based on the amino end of the catalytic domain of human Plasma Kallikrein 1B.
(Peptide available as ab43118.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody is specific for Plasma Kallikrein 1B (amino end of the catalytic domain).
We have a range of domain specific antibodies for this target. For a full list please see all Plasma Kallikrein 1B antibodies
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (see Abreview).
WB: 1/1000. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 71 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Predicted molecular weight: 71 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
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Relevance
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Plasma Kallikrein 1B is a serine proteinase of the S1 family It is secreted by the liver, kidney, adrenal gland and placenta. The zymogen is referred to as prekallikrein (PK) and is activated by Factor XIIa to an active two chain form, plasma kallikrein. In Fletcher syndrome plasma kallikrein is deficient, resulting in a prolonged partial thromboplastin time (PT).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Plasma Kallikrein 1B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Plasma Kallikrein 1B antibody images, references, products related to ab44392 and other tools.
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Plasma Kallikrein 1B antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Kallikrein 1B antibody (ab44392)
ab44392 staining Kallikrein 1B in human temporal neocortical tissue sections IHC-P . Tissue samples were fixed with formaldehyde and blocked with 10% serum for 20 minutes at 23°C: antigen retrieval was performed by heat mediation in citrate buffer.. Samples were incubated with primary antibody (1/100) for 18 hours at 23°C . A biotin-conjugated horse anti Rabbit IgG polyclonal (1/50) was used as secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to Plasma Kallikrein 1B
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Plasma Kallikrein 1B antibody - more information
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Customer reviews (feedback) regarding Plasma Kallikrein 1B antibody |
Customer FAQs regarding Plasma Kallikrein 1B antibody |
Protocols for Plasma Kallikrein 1B antibody |
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Plasma Kallikrein 1B antibody - related products:
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Plasma Kallikrein 1B antibody - other tools:
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Contact Abcam with a Technical Enquiry about Plasma Kallikrein 1B antibody |
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