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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Overview
Product name
Anti-Plasma Kallikrein 1B antibody
See all Plasma Kallikrein 1B products (7) ...
Description
Rabbit polyclonal to Plasma Kallikrein 1B
Specificity
This antibody is specific for Plasma Kallikrein 1B (amino end of the catalytic domain).
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide based on the amino end of the catalytic domain of human Plasma Kallikrein 1B.
(Peptide available as ab43118.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Kallikrein 1B antibody (ab44392)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab44392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent concentration.
IHC-P: 1/100Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/1000Predicted molecular weight: 71 kDa.(1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.)
Target
Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionP03952
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.4.21.34 antibody
- Fletcher factor antibody
- Kallikrein 3 plasma (Fletcher factor) antibody
see all
Database links
- Entrez Gene: 3818 Human
- Entrez Gene: 16621 Mouse
- Entrez Gene: 25048 Rat
- Omim: 229000 Human
- SwissProt: P03952 Human
- SwissProt: P26262 Mouse
- SwissProt: P14272 Rat
- Unigene: 237642 Human
see all
Anti-Plasma Kallikrein 1B antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Kallikrein 1B antibody (ab44392)
ab44392 staining Kallikrein 1B in human temporal neocortical tissue sections IHC-P . Tissue samples were fixed with formaldehyde and blocked with 10% serum for 20 minutes at 23°C: antigen retrieval was performed by heat mediation in citrate buffer.. Samples were incubated with primary antibody (1/100) for 18 hours at 23°C . A biotin-conjugated horse anti Rabbit IgG polyclonal (1/50) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Protocols
References for Anti-Plasma Kallikrein 1B antibody (ab44392)
ab44392 has not yet been referenced specifically in any publications.
Publishing research using ab44392? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"