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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Overview
Product name
Anti-Plasma Kallikrein 1B antibody
See all Plasma Kallikrein 1B products (7) ...
Description
Rabbit polyclonal to Plasma Kallikrein 1B
Specificity
ab75228 detects endogenous levels of fragment of activated Plasma Kallikrein 1B heavy chain resulting from cleavage adjacent to Arg390.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from human plasma Kallikrein 1B.
Positive control
Extracts from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
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Western blot - Plasma Kallikrein 1B antibody (ab75228)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 45 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionP03952
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.4.21.34 antibody
- Fletcher factor antibody
- Kallikrein 3 plasma (Fletcher factor) antibody
see all
Database links
- Entrez Gene: 3818 Human
- Omim: 229000 Human
- SwissProt: P03952 Human
- Unigene: 237642 Human
Anti-Plasma Kallikrein 1B antibody images:
Western blot - Plasma Kallikrein 1B antibody (ab75228)
All lanes : Anti-Plasma Kallikrein 1B antibody (ab75228) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HeLa cells with Cl peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 45 kDa
Observed band size : 45 kDa
References for Anti-Plasma Kallikrein 1B antibody (ab75228)
ab75228 has not yet been referenced specifically in any publications.
Publishing research using ab75228? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"