Overview

  • Product namePlasma Membrane Protein Extraction Kit
  • Assay typeDirect
  • Product overview

    The Plasma Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins), this kit was designed to not only extract the total cellular membrane proteins, but also purify the plasma membrane proteins specifically. The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

  • Tested applicationsPurificationmore details

Properties

  • RelevanceMembrane Protein Extraction is a method for extraction of membrane proteins from mammalian tissues and cells. Many procedures extract total cellular membrane proteins which is a combination of plasma and organelle membrane proteins.

Applications

Our Abpromise guarantee covers the use of ab65400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.

Protocols

References for Plasma Membrane Protein Extraction Kit (ab65400)

This product has been referenced in:
  • Yang H  et al. Inhibition of PP2A activity confers a TRAIL-sensitive phenotype during malignant transformation. Mol Cancer Res 12:217-27 (2014). Read more (PubMed: 24296757) »
  • Mehrbod P  et al. Simvastatin modulates cellular components in influenza A virus-infected cells. Int J Mol Med 34:61-73 (2014). Read more (PubMed: 24788303) »

See all 12 Publications for this product

Product Wall

This kit may be useful for extracting and retainingthe plasma membrane fraction without the plasma membrane proteins but it has not been specifically designed for this purpose. Please let us know how things go if you do choose to use ab65400 for this e...

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1. The smaller cell number can decrease yield. Hence it is at the user's discretion to reduce cell numbers. For some cells, based on size and density of expression of membrane proteins, smaller cell number can still yield enough membrane proteins. If c...

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1. This kit isolates the plasma membrane associated proteins as well as transmembrane proteins on the plasma membrane. Organelle membrane proteins are not isolated. If total membrane proteins were desired, you would get all membrane proteins ...

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Yes, this will give you two sub-cellular fractions: plasma membrane and cytosolic. So you will be able to demonstrate the relative amounts of your protein of interest in each fraction using an assay such as western blotting, assuming you have an antibo...

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The proteins isolated when using this kit should be in their native conformation.

The frozen tissue cannot be pulverized with the Dounce homogenizer. This has to be done with a pulverizer or tissue grinder. Once ground, the buffer can be used to homogenize the bits in a Dounce homogenizer.

Fresh tissue can be homogenized di...

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You are obtaining a huge pellet after step 4. This is the low speed centrifugation at 700g and pellets down unbroken cells, nuclei and debris. Getting a huge pellet at this step is indicative of incomplete homogenization. The steps are correct but...

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I would not recommend the syringe method over the Dounce homogenizer method. The Dounce method is the most gentle yet thorough way to make sure the membranes are in solution homogenously. The proteins might be damaged due to shear forces if drawn ...

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The idea is to homogenize in as much buffer as required, not too much, not too little. Our lab uses 1-2 mL of wash and homogenization buffer with 5x10^8 cells homogenizing with a dounce homogenizer for their preps to validate this kit with good results...

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Most unbound lipids are removed during the process of purifying plasma membrane proteins. This kit could work for bacteria but the bacterial cell wall has to be broken before the plasma membrane can be accessed. This can be done either by using lysozym...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"