Overview

  • Product nameAnti-Plasmin antibody (Biotin)
  • Description
    Rabbit polyclonal to Plasmin (Biotin)
  • ConjugationBiotin
  • SpecificityThis antibody does recognize plasminogen at about 20-40 %.
  • Tested applicationsRIA, WB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) - plasmin purified from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab48350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
RIA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • RelevancePlasmin is an enzyme formed in the circulating blood, and many other extracellular fluids, from the zymogen plasminogen. Plasminogen is a single-chain glycoprotein with 790 amino acid residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. Plasmin acts as a proteolytic factor in a variety of processes other than fibrinolysis; including embryonic development, tissue remodelling, tumour invasion and inflammation; in ovulation it weakens the walls of the Graafian follicle. A Deficiency of plasmin may lead to thrombosis, as clots are not degraded adequately.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Plasminogen antibody
    • PLG antibody

References for Anti-Plasmin antibody (Biotin) (ab48350)

This product has been referenced in:
  • Leksa V  et al. Dissecting mannose 6-phosphate/insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J Biol Chem : (2012). WB . Read more (PubMed: 22613725) »
  • Barker R  et al. Plasminogen and plasmin in Alzheimer's disease. Brain Res 1355:7-15 (2010). WB ; Human . Read more (PubMed: 20709033) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

We currently offer only one anti-Plasmin antibody ab48350. This product has not been tested as a neutralizing/blocking antibody for plasmin. As a rabbit polyclonal this product will recognize a number of epitop...

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Merci pour votre patience. Je m'excuse pour ce délais.

Je peux confirmer que cet anticorps était produit contre la plasmine et reconnait aussi le plasminogène à environ 20 à 40% d'intensité.

Je peux aussi confirmer que la reconnai...

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Application Western blot
Sample Human Purified protein (Blood)
Loading amount 3 µg
Specification Blood
Gel Running Conditions Reduced Denaturing (4-20% Tris HCl gels)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Rachel Barker

Verified customer

Submitted Aug 10 2009

Thank you for your enquiry. This antibody has not been tested if they can recognize plasminogen. Therefore, I can not confirm if it only detects plasmin. This antibody reacts 15% to mouse sample, which means that the remaining 85% is no reaction.

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Thank you for your enquiry. This antibody has been tested to full length human plasmin. It cross reacts with rat plasmin 10%, and mouse plasmin 15%.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"