Overview
- Product nameAnti-Plasmin antibody (Biotin)
- DescriptionRabbit polyclonal to Plasmin (Biotin)
- ConjugationBiotin
- SpecificityThis antibody does recognize plasminogen at about 20-40 %.
- Tested applicationsRIA, WB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Full length native protein (purified) - plasmin purified from human plasma.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, PBS, pH 7.5 -
Concentration information loading... - PurityProtein G purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab48350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| RIA | RIA: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent dilution. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- RelevancePlasmin is an enzyme formed in the circulating blood, and many other extracellular fluids, from the zymogen plasminogen. Plasminogen is a single-chain glycoprotein with 790 amino acid residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. Plasmin acts as a proteolytic factor in a variety of processes other than fibrinolysis; including embryonic development, tissue remodelling, tumour invasion and inflammation; in ovulation it weakens the walls of the Graafian follicle. A Deficiency of plasmin may lead to thrombosis, as clots are not degraded adequately.
- Cellular localizationSecreted
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Database links
- Entrez Gene: 5340 Human
- Omim: 173350 Human
- SwissProt: P00747 Human
- Unigene: 143436 Human
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Alternative names
- Plasminogen antibodyPLG antibody
References for Anti-Plasmin antibody (Biotin) (ab48350)
This product has been referenced in:
- Leksa V et al. Dissecting mannose 6-phosphate/insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J Biol Chem : (2012). WB . Read more (PubMed: 22613725) »
- Barker R et al. Plasminogen and plasmin in Alzheimer's disease. Brain Res 1355:7-15 (2010). WB ; Human . Read more (PubMed: 20709033) »
