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Plasminogen Activator Inhibitor 1 Human ELISA Kit (ab108891)

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Overview

  • Product namePlasminogen Activator Inhibitor 1 Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Serum, Plasma, Tissue
  • Assay typeSandwich
  • Sensitivity
    = 0.07 ng/ml
  • Range
    0.078 ng/ml - 5 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Type I plasminogen activator inhibitor (PAI-1) is a 43 kDa serpin family member that inhibits tissue- and urokinase-type plasminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA. The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogenesis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.

    Ab108891 Human PAI-1 ELISA kit is designed for detection of PAI-1 in human plasma, tissue extracts, milk, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures PAI-1 in less than 4 hours. A

    polyclonal antibody specific for PAI-1 has been pre-coated onto a microplate. PAI-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for PAI-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionSerine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in diseaseDefects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similaritiesBelongs to the serpin family.
  • Post-translational
    modifications    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P05121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Clade EEndothelial plasminogen activator inhibitorEndothelial plasminogen activator inhibitor
        NexinPAIPAIPAI 1PAI-1PAI-1PAI1_HUMANPAI1_HUMANPLANH1Plasminogen activator inhibitor 1Plasminogen activator inhibitor 1Plasminogen activator inhibitor type 1Serine (or cysteine) proteinase inhibitorSerpin E1Serpin E1Serpin peptidase inhibitor clade ESerpine 1Serpine1SERPINE1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108891 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Plasminogen Activator Inhibitor 1 Human ELISA Kit images

      • - Plasminogen Activator Inhibitor 1 Human ELISA Kit (ab108891)
        - Plasminogen Activator Inhibitor 1 Human ELISA Kit (ab108891)

      Protocols

      References for Plasminogen Activator Inhibitor 1 Human ELISA Kit (ab108891)

      ab108891 has not yet been referenced specifically in any publications.

      Publishing research using ab108891 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108891 Submit a Question for ab108891

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

      10
      Votes: Highest First

      Product code: 100662
      Lot number: GR65556-1
      Inquiry: I want to do an VEGF Elisa (ab 100662), but can not find in what anti-coagulant I have to collect the plasma in? I also ordered an Elisa kit for PAI1 (ab108891, lot# GR69250-2)and there plasma co...

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      Thank you for contacting us.

      I can confirm that Heparin should not be interfering with the assay using these kits.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information...

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      can kit be used with mouse samples?
      cross-reactivity of antibodies likely?

      Thank you for contacting us.

      I checked with the lab and they let me know that the homology with mouse is only 2%, thus they do not recommend using it for this species. It will most likely not work.

      Let me know if I can assist you find...

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      I am interested in measurements of total human and rabbit PAI-1 antigen in biological fluids. I have a question about your product ab108891 - how specific this kit is for human PAI-1? In other words, if I have a mixture of human and rabbit PAI-1, whether ...

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      Thank you for contacting Abcam regarding ab108891.


      Our human kit is specific for human PAI-1 total antigen measurement. It does not cross react with rabbit PAI-1. Unfortunately, we do not have a rabbit specific kit available.


      I...

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      I have a question about Plasminogen Activator Inhibitor 1 Human ELISA Kit (ab108891). PAI-1 is found in several forms:1.active, 2.inactive(latent), 3.complexed to tPA, 4.complexed to uPA. Which form of PAI-1 are measured in the kit ?

      Thank you for your enquiry.

      This kit measures the total PAi-1 including free PAI-1, PAI-tPA complexes and PAI-1-uPA complexes.

      I hope this information will be helpful.

      Hi Abcam,

      Could you please advise if these kits can be used to test citrate plasma?

      Thanks for your help!

      Kind regards,

      Thank you for contacting us.

      All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

      I hope this information has been of help. If your require any further information please do not he...

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      The standards for ab108891 and ab108914 di not state how much standard was in the vial. The standards were supplied as lyophillsed powder but on ab108914 the standard vial said 2ng/ml and for ab108891, the vial stated 5ng/ml. Is there 2ng and 5ng in these...

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      Thank you for contacting Abcam today.

      I am sorry about the confusion over the labeling of the standards for ab108891 and ab108914. I have heard back from the lab about the correct concentrations for these standards:

      ab108891 - Reconsti...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"