Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894)

Overview

  • Product namePlasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeQuantitative
  • Sensitivity
    0.6 IU/ml
  • Range
    0.625 IU/ml - 20 IU/ml
  • Product overview

    Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA. The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogensis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.

    Ab108894 Human PAI-1 Chromogenic Activity Assay Kit is developed to determine human PAI-1 activity in cell culture supernatants. A fixed amount of tPA is added in excess to undiluted sample, which allows PAI-1 and tPA to form an inactive complex. The assay measures plasminogen activation by residual tPA in coupled assays that contain tPA, plasminogen, and a plsmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The absorbance of the pNA at 405 nm is inversely proportional to the PAI-1 enzymatic activity.

  • Tested applicationsFunctional Studiesmore details

Properties

  • Alternative names
    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpine 1
    • Serpine1
    see all

Applications

Our Abpromise guarantee covers the use of ab108894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit images

Protocols

References for Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894)

ab108894 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"