Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894)

Overview

  • Product namePlasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeQuantitative
  • Sensitivity
    0.5 IU/ml
  • Range
    0.625 IU/ml - 20 IU/ml
  • Product overview

    Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894) has been developed to determine human type I plasminogen activator inhibitor (PAI-1) activity in cell culture supernatants. A fixed amount of tissue-type plasminogen activator (tPA) is added in excess to undiluted sample, which allows PAI-1 and tPA to form an inactive complex. The assay measures plasminogen activation by residual tPA in coupled assays that contain tPA, plasminogen, and a plsmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The absorbance of the pNA at 405 nm is inversely proportional to the PAI-1 enzymatic activity.

  • Notes

    Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA. The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogensis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108894 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent dilution.

    Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit images

    Protocols

    References for Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894)

    ab108894 has not yet been referenced specifically in any publications.

    Product Wall

    Our lab has found that rat and mouse samples both show no significant activity with this particular kit. We would not advise using mouse or rat samples.

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    Todas las referencias, publicaciones, e información disponible sobre nuestros productos la compartimos en nuestra web para utilidad de los usuarios.
    Lamentablemente para este kit no tenemos ninguna referencia ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"