Anti-Plasminogen antibody (ab154560)
Key features and details
- Rabbit polyclonal to Plasminogen
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Plasminogen antibody
See all Plasminogen primary antibodies -
Description
Rabbit polyclonal to Plasminogen -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 84-434 of Human Plasminogen (Uniprot ID: P00747).
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Positive control
- Jurkat, Raji, K562, BCL1, PC12 and Rat2 whole cell lysates, HeLa cells, Human C2C12 xenograft tissue and hepatoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 90 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 90 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
Tissue specificity
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
Involvement in disease
Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Domain
Kringle domains mediate interaction with CSPG4. -
Post-translational
modificationsN-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
Cellular localization
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 5340 Human
- Entrez Gene: 18815 Mouse
- Entrez Gene: 85253 Rat
- Omim: 173350 Human
- SwissProt: P00747 Human
- SwissProt: P20918 Mouse
- SwissProt: Q01177 Rat
- Unigene: 143436 Human
see all -
Form
Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B -
Alternative names
- Plasmin antibody
- Plasmin heavy chain A antibody
- Plasmin light chain B antibody
see all
Images
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Anti-Plasminogen antibody (ab154560) at 1/1000 dilution + BCL1 whole cell lysate at 30 µg
Predicted band size: 90 kDa
7.5% SDS PAGE -
All lanes : Anti-Plasminogen antibody (ab154560) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Rat prostate tissue, labeling Plasminogen using ab154560 at a 1/500 dilution.
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All lanes : Anti-Plasminogen antibody (ab154560) at 1/1000 dilution
Lane 1 : PC12 whole cell lysate
Lane 2 : Rat2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Plasminogen using ab154560 at a 1/500 dilution. Lower image is costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human C2C12 xenograft, labeling Plasminogen using ab154560 at a 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded human Hepatoma tissue, labeling Plasminogen using ab154560 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab154560 has been referenced in 13 publications.
- Yu JE et al. Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization. Mol Oncol 16:2214-2234 (2022). PubMed: 34861103
- Hu PA et al. New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation. Nutrients 14:N/A (2022). PubMed: 35684129
- Bai X et al. Analysis of transcriptome characteristics of UTI therapy for cerebral injury after CA/ROSC based on RNA-seq technique. Iran J Basic Med Sci 25:715-722 (2022). PubMed: 35949311
- Xiao M et al. Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome. Acta Physiol (Oxf) 231:e13512 (2021). PubMed: 32455507
- Andersen H et al. Plasminogen Deficiency and Amiloride Mitigate Angiotensin II-Induced Hypertension in Type 1 Diabetic Mice Suggesting Effects Through the Epithelial Sodium Channel. J Am Heart Assoc 9:e016387 (2020). PubMed: 33215566