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Anti-Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)

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Overview

Product name

Anti-Plastin L antibody [LPL4A.1] - BSA and Azide free
See all Plastin L products (7) ...

Description

Mouse monoclonal [LPL4A.1] to Plastin L - BSA and Azide free

Specificity

ab80729 recognizes Plastin L in a Ca2+ independent fashion but does not react with T- or I- Plastin. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested applications

WB, IP, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Human recombinant Plastin L protein

Epitope

Headpiece region of Plastin L

Positive control

Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 10mM PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

LPL4A.1

Isotype

IgG1

Research areas

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

  • Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab80729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Fixed in methanol or 3% paraformaldehyde. Use at an assay dependent dilution.
IP: Use at 2µg/mg of lysate.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.

Tissue specificity

Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.

Sequence similarities

Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.

Post-translational
modifications

Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked.

Cellular localization

Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.

Target information above from: UniProt accessionP13796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • L plastin antibody
  • L-plastin antibody
  • LC64P antibody
  • LCP 1 antibody
  • LCP-1 antibody
  • LCP1 antibody
  • LPL antibody
  • Lplastin antibody
  • Lymphocyte cytosolic protein 1 antibody
  • Plastin 2 antibody
  • Plastin-2 antibody
  • Plastin2 antibody
  • PlastinL antibody
  • PLS2 antibody
  • PLSL antibody
  • PLSL_HUMAN antibody
see all

Database links

Anti-Plastin L antibody [LPL4A.1] - BSA and Azide free images:

  Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)

Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)

Anti-Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729) at 1 µg/ml + Human Cells-15 Lysate

Predicted band size : 70 kDa
Observed band size : 70 kDa

References for Anti-Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)

This product has been referenced in:

  • Lollike Ket al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001).Read more (PubMed: 11279160) »
  • Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem 271:14623-30 (1996).Read more (PubMed: 8663066) »

See all 2 publications for this product

Publishing research using ab80729? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"