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Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)
![Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)](/ps/datasheet/images/80/ab80729/Plastin-L-Primary-antibodies-ab80729-1.jpg)
(enlarge)
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Product Name
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Plastin L antibody [LPL4A.1] - BSA and Azide free
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See all Plastin L antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [LPL4A.1] to Plastin L - BSA and Azide free
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Immunogen
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Human recombinant Plastin L protein
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Specificity
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ab80729 recognizes Plastin L in a Ca2+ independent fashion but does not react with T- or I- Plastin.
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Epitope
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Headpiece region of Plastin L
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC/IF: Fixed in methanol or 3% paraformaldehyde. Use at an assay dependent dilution. IP: Use at 2µg/mg of lysate. WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Tumor biomarkers >> Tumor antigens Cancer >> Tumor immunology >> Tumor-associated antigens Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Lipoprotein lipase (LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion. Recently, it has also been shown that LPL promote the development of atherosclerosis through facilitation of monocyte adhesion to endothelial cells, stimulation of tumor necrosis factor alpha (TNF ) secretion and induction of vascular smooth muscle cell proliferation.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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LPL4A.1
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None Constituents: 10mM PBS, pH 7.4
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Plastin L antibody [LPL4A.1] - BSA and Azide free is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Plastin L antibody [LPL4A.1] - BSA and Azide free images, references, products related to ab80729 and other tools.
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Plastin L antibody [LPL4A.1] - BSA and Azide free images:
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Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)
Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729) at 1 µg/ml + Human Cells-15 Lysate
Predicted band size : 70 kDa Observed band size : 70 kDa
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References for Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)
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This clone has been used in: (two most recent references)
Lollike K et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001). PubMed: 11279160
Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem 271:14623-30 (1996). PubMed: 8663066
If you publish research using ab80729 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Plastin L
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Plastin L antibody [LPL4A.1] - BSA and Azide free - more information
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