Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)

Product Name 

Plastin L antibody [LPL4A.1] - BSA and Azide free

Product type 

Primary antibodies

Description 

Mouse monoclonal [LPL4A.1] to Plastin L - BSA and Azide free

Immunogen 

Human recombinant Plastin L protein

Reacts with 

Hu

Does not react with 
(species key)

Ms

Specificity 

ab80729 recognizes Plastin L in a Ca²⁺ independent fashion but does not react with T- or I- Plastin.

Epitope 

Headpiece region of Plastin L

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Fixed in methanol or 3% paraformaldehyde. Use at an assay dependent dilution.
IP: Use at 2µg/mg of lysate.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

Relevance 

Lipoprotein lipase (LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion. Recently, it has also been shown that LPL promote the development of atherosclerosis through facilitation of monocyte adhesion to endothelial cells, stimulation of tumor necrosis factor alpha (TNF ) secretion and induction of vascular smooth muscle cell proliferation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

LPL4A.1

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: 10mM PBS, pH 7.4

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

See below for Plastin L antibody [LPL4A.1] - BSA and Azide free images, references, products related to ab80729 and other tools.

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References for Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)

  This clone has been used in: (two most recent references)

Lollike K et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001). PubMed: 11279160

Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem 271:14623-30 (1996). PubMed: 8663066
If you publish research using ab80729 please let us know so that we can cite the reference on this datasheet.

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