Plastin L antibody [LPL4A.1] (ab3290)

Product Name 

Plastin L antibody [LPL4A.1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [LPL4A.1] to Plastin L

Immunogen 

Recombinant full length protein (Human).

Reacts with 

Hu

Does not react with 
(species key)

Ms

Specificity 

This antibody does not react with T or I plastin.
It recognizes LPL in Calcium independent fashion.

Epitope 

Headpiece region.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IF: Use at an assay dependent concentration.
IHC-Fr: 1/50.
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human polymorphonuclear neutrophils / monocytes, MCF 7, and Jurkat cells.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

Relevance 

Lipoprotein lipase (LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion. Recently, it has also been shown that LPL promote the development of atherosclerosis through facilitation of monocyte adhesion to endothelial cells, stimulation of tumor necrosis factor alpha (TNF ) secretion and induction of vascular smooth muscle cell proliferation.

L plastin is a leukocyte specific actin bundling protein from the fimbrin family of proteins. It has two actin binding domains and two EF hand type calcium binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L plastin may play a role in regulating integrin mediated adhesion in leukocytes. L plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosterone in androgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

LPL4A.1

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

References for Plastin L antibody [LPL4A.1] (ab3290)

  This product has been used in:

Lollike K et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001). PubMed: 11279160
If you publish research using ab3290 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Plastin L antibody [LPL4A.1]

Customer FAQs regarding Plastin L antibody [LPL4A.1]

Protocols for Plastin L antibody [LPL4A.1]

Price and availability of products related to Plastin L antibody [LPL4A.1]

Plastin L antibody [LPL4A.1] - related products: