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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Plastin L antibody
See all Plastin L products (7) ...
Description
Rabbit polyclonal to Plastin L
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide: LNILEEIGGG QKVNDDIIVN WVNETLREAK KSSSISSFKD PKISTSLPVL, corresponding to a region within internal sequence amino acids 504 - 553 of Human Plastin L (NP_002289)
Positive control
Placenta lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Western blot - Plastin L antibody (ab81454)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 70 kDa.
Target
Function
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Tissue specificity
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
Sequence similarities
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Post-translational
modifications
Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked.
Cellular localization
Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
Target information above from: UniProt accessionP13796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- L plastin antibody
- L-plastin antibody
- LC64P antibody
see all
Database links
- Entrez Gene: 3936 Human
- Entrez Gene: 18826 Mouse
- Entrez Gene: 306071 Rat
- Entrez Gene: 30583 Zebrafish
- Omim: 153430 Human
- SwissProt: P13796 Human
- SwissProt: Q61233 Mouse
- SwissProt: Q6P698 Zebrafish
see all
Anti-Plastin L antibody images:
Western blot - Plastin L antibody (ab81454)
Anti-Plastin L antibody (ab81454) at 1 µg/ml (in 5% skim milk / PBS buffer) + placenta lysate at 10 µg/ml
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 70 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-Plastin L antibody (ab81454)
ab81454 has not yet been referenced specifically in any publications.
Publishing research using ab81454? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"