Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Plastin L antibody
See all Plastin L products (7) ...
Description
Rabbit polyclonal to Plastin L
Tested applications
WB, IP, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to the C terminal of Human Plastin L protein.
Positive control
HL60 cell lysate. Mouse spleen lysate. HL60 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS containing antibody stabilizer, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1.0 µg/ml.Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP: Use a concentration of 2 - 5 µg/ml.
ELISA: Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P: Use a concentration of 0.5 - 2.0 µg/ml.
Target
Function
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Tissue specificity
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
Sequence similarities
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Post-translational
modifications
Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked.
Cellular localization
Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
Target information above from: UniProt accessionP13796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- L plastin antibody
- L-plastin antibody
- LC64P antibody
see all
Database links
- Entrez Gene: 3936 Human
- Entrez Gene: 18826 Mouse
- Entrez Gene: 306071 Rat
- Omim: 153430 Human
- SwissProt: P13796 Human
- SwissProt: Q61233 Mouse
- Unigene: 381099 Human
- Unigene: 710563 Human
see all
Anti-Plastin L antibody images:
Western blot - Plastin L antibody (ab83496)
All lanes : Anti-Plastin L antibody (ab83496) at 1/500 dilution
Lane 1 : HL60 cell lysate
Lane 2 : Mouse spleen lysate
Predicted band size : 70 kDa
Observed band size : 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plastin L antibody (ab83496)
ab83496 at a 1/200 dilution, staining Plastin L in HL60 cells.
References for Anti-Plastin L antibody (ab83496)
ab83496 has not yet been referenced specifically in any publications.
Publishing research using ab83496? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"