Platelet Glycoprotein IIb + IIIa Human ELISA Kit (ab108851)
- Product namePlatelet Glycoprotein IIb + IIIa Human ELISA Kit
- Tests1 x 96 well plate
- Sample typePlasma, Platelets, Cell culture extracts
- Assay typeSandwich
- Sensitivity= 1 ng/ml
- Range1.25 ng/ml - 40 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Platelet Glycoprotein IIb + IIIa is a member of the integrin family of cell membrane receptors that play key roles in thrombus formation, platelet aggregation, embryogenesis and intercellular adhesion. Each integrin receptor complex consists of a heavy (alpha) and a light (beta) chain associated as a calcium-dependent heterodimer with a molecular mass of 140 kDa and 90 kDa respectively.
ab108851 Platelet Glycoprotein IIb + IIIa Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Platelet Glycoprotein IIb + IIIa in platelets, platelet-rich plasma and cell culture lysate. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Platelet Glycoprotein IIb + IIIa in less than 4 hours. A polyclonal antibody specific for Human Platelet Glycoprotein IIb + IIIa has been pre-coated onto a 96-well microplate with removable strips. Platelet Glycoprotein IIb + IIIa in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for Platelet Glycoprotein IIb + IIIa which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Platelet Glycoprotein IIb + IIIa Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Platelet Glycoprotein IIb + IIIa Microplate 1 x 96 well 1 x 96 tests Platelet Glycoprotein IIb + IIIa Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- GPIIb/IIIaIntegrin alpha IIb beta 3Platelet membrane glycoprotein IIb/IIIa
Our Abpromise guarantee covers the use of ab108851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Platelet Glycoprotein IIb + IIIa Human ELISA Kit images
Platelet Glycoprotein IIb + IIIa Standard Curve
References for Platelet Glycoprotein IIb + IIIa Human ELISA Kit (ab108851)
ab108851 has not yet been referenced specifically in any publications.