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Products:Cardiovascular >> Vasculature >> Endothelium
Overview
Product name
Anti-Plexin D1 antibody
See all Plexin D1 products (3) ...
Description
Goat polyclonal to Plexin D1
Tested applications
ICC/IF, IP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Cow, Dog, Pig
Immunogen
Synthetic peptide: LAEPKKSHRQSH, corresponding to amino acids 1683-1694 of Human Plexin D1
LAEPKKSHRQ SH
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plexin D1 antibody (ab28762)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 20385769)
IP: Use at an assay dependent concentration. (PubMed: 20385769)
WB: 1/1000
IHC-P: 1/300Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Tissue specificity
Detected at low levels in heart, placenta, lung, skeletal muscle, kidney, thymus and liver. Detected at very low levels in brain, colon, spleen, small intestine and peripheral blood leukocytes.
Sequence similarities
Belongs to the plexin family.
Contains 3 IPT/TIG domains.
Contains 1 Sema domain.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ9Y4D7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- KIAA0620 antibody
- MGC75353 antibody
- PLEX D1 antibody
see all
Database links
- Entrez Gene: 23129 Human
- Entrez Gene: 67784 Mouse
- Omim: 604282 Human
- SwissProt: Q9Y4D7 Human
- SwissProt: Q3UH93 Mouse
- SwissProt: Q68HV1 Mouse
- SwissProt: Q8VCL8 Mouse
- SwissProt: Q9CZF2 Mouse
see all
Anti-Plexin D1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plexin D1 antibody (ab28762)
ab28762 at 1/300 dilution, staining Plexin D1 in mouse brain tissue sections by IHC-P (Formalin / PFA fixed prafaffin embedded sections). Tissue underwent formaldehyde fixation, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocked in 5% serum for 1 hour. The primary antibody was used at 1/300 dillution for 1 hour. A Biotin-conjugated rabbit polyclonal to goat IgG was used as secondary at 1/250 dillution.
References for Anti-Plexin D1 antibody (ab28762)
This product has been referenced in:
- Parikh Tet al. Proteomic analyses of corneal tissue subjected to alkali exposure. Invest Ophthalmol Vis Sci 52:1819-31 (2011). WB.Read more (PubMed: 20861482) »
- Sakurai Aet al. Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras. Mol Cell Biol 30:3086-98 (2010). WB, ICC/IF, IP; Human, Mouse, African Green Monkey.Read more (PubMed: 20385769) »
See all 2 publications for this product
Publishing research using ab28762? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"