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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with
Human Plzf peptide (ab39353).
Information by UniProt
Anti-Plzf antibody images
Western blot - Plzf antibody (ab39354)
All lanes : Anti-Plzf antibody (ab39354) at 1 µg/ml Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 75 kDa Observed band size : 85 kDa ( why is the actual band size different from the predicted?) Exposure time : 15 minutes
Western blot - Anti-Plzf antibody (ab39354)
All lanes : Anti-Plzf antibody (ab39354) at 1.25 µg/ml Lane 1 : Marker Lane 2 : Zebrafish brain homogenate at 20 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/10000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 75 kDa Observed band size : 87 kDa ( why is the actual band size different from the predicted?) Exposure time : 5 minutes
References for Anti-Plzf antibody (ab39354)
This product has been referenced in:
et al. The (pro)renin receptor mediates constitutive PLZF-independent pro-proliferative effects which are inhibited by bafilomycin but not genistein. Int J Mol Med 33:795-808 (2014).
Read more (PubMed: 24424509) »
et al. 8-CPT-cAMP/all-trans retinoic acid targets t(11;17) acute promyelocytic leukemia through enhanced cell differentiation and PLZF/RARa degradation. Proc Natl Acad Sci U S A 110:3495-500 (2013).
Read more (PubMed: 23382200) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"