Overview

  • Product nameAnti-Plzf antibody
    See all Plzf primary antibodies
  • Description
    Rabbit polyclonal to Plzf
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Plzf.

    (Peptide available as ab39353.)

  • Positive control
    • This antibody gave a positive signal in HL60 and Ramos whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab39354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353).

Target

Anti-Plzf antibody images

  • All lanes : Anti-Plzf antibody (ab39354) at 1 µg/ml

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes
  • All lanes : Anti-Plzf antibody (ab39354) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 87 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-Plzf antibody (ab39354)

This product has been referenced in:
  • Kirsch S  et al. The (pro)renin receptor mediates constitutive PLZF-independent pro-proliferative effects which are inhibited by bafilomycin but not genistein. Int J Mol Med 33:795-808 (2014). WB . Read more (PubMed: 24424509) »
  • Jiao B  et al. 8-CPT-cAMP/all-trans retinoic acid targets t(11;17) acute promyelocytic leukemia through enhanced cell differentiation and PLZF/RARa degradation. Proc Natl Acad Sci U S A 110:3495-500 (2013). WB ; Mouse . Read more (PubMed: 23382200) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Adult marmoset testis)
Specification Adult marmoset testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Zachary Yu-Ching Lin

Verified customer

Submitted Dec 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Adult mouse testis)
Specification Adult mouse testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Zachary Yu-Ching Lin

Verified customer

Submitted Nov 20 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Paraformaldehyde
Permeabilization Yes - 0.1 % Trition in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"