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Product name
Anti-Podoplanin / gp36 antibody
See all Podoplanin / gp36 products (17) ...
Description
Hamster monoclonal to Podoplanin / gp36
Specificity
The monoclonal antibody will detect podoplanin on the surface of mouse lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry.
Tested applications
IHC-Fr, IHC-P, IP, WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse
Does not react with
Human
Immunogen
Monoclonal antibodies were produced by immunizing Syrian hamsters with a murine thymic stromal cell line.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from cell culture supernatant.
Clonality
Monoclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Endothelium
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Cardiovascular >> Blood >> Other
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody (ab11936)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IP
Flow Cyt
Application notes
Flow Cyt: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution (PMID 18829396).
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
Tissue specificity
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
Sequence similarities
Belongs to the podoplanin family.
Post-translational
modifications
Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked.
Cellular localization
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
Target information above from: UniProt accessionQ86YL7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AGGRUS antibody
- GLYCOPROTEIN 36 KD antibody
- Glycoprotein 36 antibody
see all
Database links
- Entrez Gene: 14726 Mouse
- SwissProt: Q62011 Mouse
- Unigene: 2976 Mouse
Anti-Podoplanin / gp36 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody (ab11936)
ab11936 staining Podoplanin / gp36 in murine skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an enzymatic antigen retrieval step was performed using Protein K. Samples were then blocked with 5% serum for 30 minutes at room temperature and then incubated with ab11936 at a 1/400 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-hamster IgG used at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-Podoplanin / gp36 antibody (ab11936)
This product has been referenced in:
- Brown K et al. Tertiary lymphoid organs in renal allografts can be associated with donor-specific tolerance rather than rejection. Eur J Immunol 41:89-96 (2011).Read more (PubMed: 21182080) »
- Grimaldo S et al. Specific role of lymphatic marker podoplanin in retinal pigment epithelial cells. Lymphology 43:128-34 (2010).Read more (PubMed: 21226415) »
See all 10 publications for this product
Publishing research using ab11936? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"