Anti-Polyoma virus, Large T antigen antibody [PyLT] (ab18187)
Key features and details
- Rat monoclonal [PyLT] to Polyoma virus, Large T antigen
- Suitable for: WB, IP, ICC/IF
- Isotype: IgG2b
Overview
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Product name
Anti-Polyoma virus, Large T antigen antibody [PyLT]
See all Polyoma virus, Large T antigen primary antibodies -
Description
Rat monoclonal [PyLT] to Polyoma virus, Large T antigen -
Host species
Rat -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Polyomavirus -
Immunogen
Tissue, cells or virus corresponding to Polyoma virus, Large T antigen. Polyoma virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
PyLT -
Myeloma
NS1 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution. Detects a band of approximately 100 kDa.
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IP |
Use at an assay dependent dilution.
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ICC/IF |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. Detects a band of approximately 100 kDa. |
IP
Use at an assay dependent dilution. |
ICC/IF
Use at an assay dependent dilution. |
Target
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Relevance
The mouse polyoma virus (Py) is an oncogenic virus, which encodes for three early proteins, large, middle and small T (tumour) antigen. PyLT allows isolation of viral T antigens.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab18187 has been referenced in 2 publications.
- Dilworth SM Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen. EMBO J 1:1319-28 (1982). PubMed: 6327264
- Dilworth SM & Griffin BE Monoclonal antibodies against polyoma virus tumor antigens. Proc Natl Acad Sci U S A 79:1059-63 (1982). PubMed: 6175960