RabMAb

Anti-Prealbumin antibody [EP2929Y] (ab75815)

Overview

  • Product nameAnti-Prealbumin antibody [EP2929Y]
    See all Prealbumin primary antibodies
  • Description
    Rabbit monoclonal [EP2929Y] to Prealbumin
  • Tested applicationsWB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues on the C-terminus of human Prealbumin

  • Positive control
    • Human heart, placenta and brain lysates; human liver tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab75815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP 1/60.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100.
Flow Cyt 1/50.

Target

  • FunctionThyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • Tissue specificityDetected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • Involvement in diseaseDefects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • Sequence similaritiesBelongs to the transthyretin family.
  • DomainEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • Cellular localizationSecreted. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amyloid polyneuropathy antibody
    • Amyloidosis I antibody
    • ATTR antibody
    • Carpal tunnel syndrome 1 antibody
    • CTS antibody
    • CTS1 antibody
    • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
    • Dystransthyretinemic hyperthyroxinemia antibody
    • Epididymis luminal protein 111 antibody
    • HEL111 antibody
    • HsT2651 antibody
    • PALB antibody
    • Prealbumin amyloidosis type I antibody
    • Prealbumin antibody
    • Prealbumin Thyroxine-binding antibody
    • Senile systemic amyloidosis antibody
    • TBPA antibody
    • Thyroxine binding prealbumin antibody
    • Transthyretin antibody
    • TTHY_HUMAN antibody
    • TTR antibody
    • TTR protein antibody
    see all

Anti-Prealbumin antibody [EP2929Y] images

  • ab75815 at 1/250 dilution staining Prealbumin in human liver by Immunohistochemistry, Paraffin-embedded tissue.
  • All lanes : Anti-Prealbumin antibody [EP2929Y] (ab75815) at 1/5000 dilution

    Lane 1 : human heart lysate
    Lane 2 : human placenta lysate
    Lane 3 : human brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa

References for Anti-Prealbumin antibody [EP2929Y] (ab75815)

ab75815 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Human Serum (cerebrospinal fluid from human subjects)
Specification cerebrospinal fluid from human subjects
Blocking step Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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Verified customer

Submitted Sep 08 2014

Thank you for your inquiry.

Unfortunately, ab75815, ab92469, and ab133284 (2434-1, 2524-1, 3168-1) were not raised from a region between a.a. 105 - 115. I took a look at the rest of the prealbumin antibodies in our catalogue and sadly none we...

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