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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Overview
Product name
Anti-Prealbumin antibody
See all Prealbumin products (12) ...
Description
Rabbit polyclonal to Prealbumin
Tested applications
IHC-P, RID, WBmore details
Cross reactivity
Reacts with
Sheep, Goat, Horse, Chicken, Cow, Cat, Dog, Human
Does not react with
Mouse, Rat, Guinea pig, Pig, Kangaroo, Mink (Mustela)
Immunogen
Prealbumin isolated from human plasma.
Positive control
Whole plasma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.1M Sodium chloride
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This "Immunoglobulin Fraction" is purified by four purification steps: A) Ammonium sulphate precipitation, B) Lipoprotein dialysis to remove lipoproteins, C) DEAE-anion exchange chromatography, and D) a second ammonium sulphate precipitaiton. The purified immunoglobulin fraction is then "Specificity Purified" by solid phase absorption which serves to remove possible trace amounts of unspecific immunoglobulins from the antibody. A solid phase absorption is performed by passing the antibody through a Sepharose solid matrix coupled with human serum proteins that bind the unspecific immunoglobulins in the antibody by an antigen-antibody reaction. This process ensures that the antibody is specific (i.e. it reacts only with Prealbumin and no other human proteins).
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Serum Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
RID: Use at an assay dependent dilution. (Rocket Immunoelectrophoresis: Use 0.6 µL per square cm gel area. Crossed Immunoelectrophoresis: Only the prealbumin precipitation arch appears when using 12.5 µL Ab16006 per square cm gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue. Gel Precipitation techniques: Use at an assay dependent dilution.)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).(Not suitable for turbidimetry and nephelometry.)
Target
Function
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Tissue specificity
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
Involvement in disease
Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
Sequence similarities
Belongs to the transthyretin family.
Domain
Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
Cellular localization
Secreted. Cytoplasm.
Target information above from: UniProt accessionP02766
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amyloid polyneuropathy antibody
- Amyloidosis I antibody
- ATTR antibody
see all
Database links
- Entrez Gene: 396277 Chicken
- Entrez Gene: 280948 Cow
- Entrez Gene: 7276 Human
- Omim: 176300 Human
- SwissProt: P27731 Chicken
- SwissProt: O46375 Cow
- SwissProt: P02766 Human
- SwissProt: P12303 Sheep
see all
Anti-Prealbumin antibody images:
Western blot - Prealbumin antibody (ab16006)
Anti-Prealbumin antibody (ab16006) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 16 kDa
Observed band size : 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prealbumin antibody (ab16006)
ab55811 staining Prealbumin in human liver.
Left panel: with primary antibody at 2 µg/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Prealbumin antibody (ab16006)
This product has been referenced in:
- Holzer Met al. Uremia Alters HDL Composition and Function. J Am Soc Nephrol 22:1631-41 (2011). WB; Human.Read more (PubMed: 21804091) »
- Saminathan Ret al. VKORC1 pharmacogenetics and pharmacoproteomics in patients on warfarin anticoagulant therapy: transthyretin precursor as a potential biomarker. PLoS One 5:e15064 (2010). WB; Human.Read more (PubMed: 21179214) »
See all 2 publications for this product
Publishing research using ab16006? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"