Anti-Prealbumin antibody (ab78548)
- Product nameAnti-Prealbumin antibodySee all Prealbumin primary antibodies ...
- DescriptionRabbit polyclonal to Prealbumin
- Tested applicationsIHC-P, ICC/IF, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Pig, Chimpanzee, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Prealbumin.
(Peptide available as ab94546.)
- Positive control
- This antibody gave a positive signal in human plasma total protein tissue lysate. IF/ICC: MCF7 cell line.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab78548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use a concentration of 1 µg/ml.|
|ICC/IF||ICC/IF: Use a concentration of 5 µg/ml.|
|WB||WB: Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).Can be blocked with Prealbumin peptide (ab94546).|
- FunctionThyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
- Tissue specificityDetected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
- Involvement in diseaseDefects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
- Sequence similaritiesBelongs to the transthyretin family.
- DomainEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
- Cellular localizationSecreted. Cytoplasm.
- Amyloid polyneuropathy antibody
- Amyloidosis I antibody
- ATTR antibody
- Carpal tunnel syndrome 1 antibody
- CTS antibody
- CTS1 antibody
- Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
- Dystransthyretinemic hyperthyroxinemia antibody
- HsT2651 antibody
- PALB antibody
- Prealbumin amyloidosis type I antibody
- Prealbumin antibody
- Senile systemic amyloidosis antibody
- TBPA antibody
- Thyroxine binding prealbumin antibody
- Transthyretin antibody
- TTHY_HUMAN antibody
- TTR antibody
- TTR protein antibody
Anti-Prealbumin antibody images
ICC/IF image of ab78548 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78548, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Anti-Prealbumin antibody (ab78548) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 16 kDa
Exposure time : 3 minutes
IHC image of Prealbumin staining in Human Normal Liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78548, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
References for Anti-Prealbumin antibody (ab78548)
ab78548 has not yet been referenced specifically in any publications.