Overview

  • Product nameAnti-Prealbumin antibodySee all Prealbumin primary antibodies ...
  • Description
    Sheep polyclonal to Prealbumin
  • SpecificityThis product gives a single arc when tested by IEP and 2D-IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human Prealbumin.
  • Tested applicationsIHC-P, RID, Immunoelectrophoresis, Double Immunodiffusion, WB, ICC/IF, Othermore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Immunogen

    Human prealbumin purified from plasma.

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferGlycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAntiserum is prepared by immunisation of sheep with Human Prealbumin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
  • Clonality Polyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
RID Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
Double Immunodiffusion Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
ICC/IF Use a concentration of 5 µg/ml.
Other Use at an assay dependent concentration.

Target

  • FunctionThyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • Tissue specificityDetected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • Involvement in diseaseDefects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • Sequence similaritiesBelongs to the transthyretin family.
  • DomainEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • Cellular localizationSecreted. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amyloid polyneuropathy antibody
    • Amyloidosis I antibody
    • ATTR antibody
    • Carpal tunnel syndrome 1 antibody
    • CTS antibody
    • CTS1 antibody
    • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
    • Dystransthyretinemic hyperthyroxinemia antibody
    • Epididymis luminal protein 111 antibody
    • HEL111 antibody
    • HsT2651 antibody
    • PALB antibody
    • Prealbumin amyloidosis type I antibody
    • Prealbumin antibody
    • Prealbumin Thyroxine-binding antibody
    • Senile systemic amyloidosis antibody
    • TBPA antibody
    • Thyroxine binding prealbumin antibody
    • Transthyretin antibody
    • TTHY_HUMAN antibody
    • TTR antibody
    • TTR protein antibody
    see all

Anti-Prealbumin antibody images

  • ICC/IF image of ab9015 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9015 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Prealbumin antibody (ab9015)

This product has been referenced in:
  • Hibert P  et al. Modifications in rat plasma proteome after remote ischemic preconditioning (RIPC) stimulus: identification by a SELDI-TOF-MS approach. PLoS One 9:e85669 (2014). Read more (PubMed: 24454915) »
  • Brouillette J  et al. Neurotoxicity and memory deficits induced by soluble low-molecular-weight amyloid-ß1-42 oligomers are revealed in vivo by using a novel animal model. J Neurosci 32:7852-61 (2012). Mouse . Read more (PubMed: 22674261) »

See all 8 Publications for this product

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It has not been specifically tested on frozen sections. However, since it is suitable for formalin-fixed paraffin embedded tissues, it is very likely that it works on frozen sections as well.

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Thank you for your enquiry and your interest in our products.

I can confirm that ab9015 has been tested and characterized in IHC-P, RID, Immunoelectrophoresis, Double Immunodiffusion, WB and it recognizes pig (porcine) Prealbumin.

I...

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Application Western blot
Sample Human Cell lysate - whole cell (human blood serum)
Loading amount 78 µg
Specification human blood serum
Gel Running Conditions Reduced Denaturing (15%, 6V, 45 min.)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Mar 10 2010

Application Western blot
Sample Mouse Tissue lysate - other (serum)
Specification serum
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 30 2007

Application Western blot
Sample Rat Cell lysate - other (serum)
Loading amount 40 µg
Specification serum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Apr 11 2006

The antibody has not been tested in western blotting yet. According to SwissProt the predicted MW of prealbumin is around 15kDa. I hope this information helps,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"