Anti-Presenilin 2 antibody [APS 21] (ab15548)

Overview

• Product nameAnti-Presenilin 2 antibody [APS 21]See all Presenilin 2 primary antibodies ...
• Description
  Mouse monoclonal [APS 21] to Presenilin 2
• SpecificityNo cross-reactivity is seen with presenilin 1.
• Tested applicationsWB, ICC/IF, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide: CQEGRQGPEDGENTA, corresponding to amino acids 31-45 of Human Presenilin 2 with N-terminally added cysteine.

  Run BLAST with Run BLAST with
• Positive controlPresenilin 2 transfected COS-7 cells.

Properties

• FormLiquid
• Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, 1mg/ml BSA
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberAPS 21
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Proteases

  • Neuroscience
  • Neurology process
  • Notch Pathway

Applications

Target

• FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
• Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
• Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
  Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
• Sequence similaritiesBelongs to the peptidase A22A family.
• DomainThe PAL motif is required for normal active site conformation.
• Post-translational
  modificationsHeterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
  Phosphorylated on serine residues.
• Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.

Target information above from: UniProt accession P49810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5664 Human
  • Entrez Gene: 19165 Mouse
  • Entrez Gene: 81751 Rat
  • Omim: 600759 Human
  • SwissProt: P49810 Human
  • SwissProt: Q61144 Mouse
  • SwissProt: O88777 Rat
  • Unigene: 25363 Human

  • Unigene: 330850 Mouse
  • Unigene: 11045 Rat

  see all

• Alternative names
  AD 4 antibodyAD 4 antibodyAD3L antibody

  AD3L antibodyAD3LP antibodyAD3LP antibodyAD4 antibodyAD4 antibodyAD5 antibodyAD5 antibodyAlzheimer disease 4 antibodyAlzheimer disease 4 antibodyAlzheimer Disease Familial Type 4 antibodyAlzheimer Disease Familial Type 4 antibodyAlzheimer's disease 3 like antibodyAlzheimer's disease 3 like antibodyCMD1V antibodyE5 1 antibodyE5 1 antibodyE5-1 antibodyOTTHUMP00000035671 antibodyOTTHUMP00000035672 antibodyOTTHUMP00000228286 antibodyOTTHUMP00000228288 antibodyPresenilin 2 (Alzheimer disease 4) antibodyPresenilin 2 antibodyPresenilin-2 CTF subunit antibodyPS 2 antibodyPS 2 antibodyPS-2 antibodyPS2 antibodyPS2 antibodyPSEN 2 antibodyPSEN 2 antibodyPSEN2 antibodyPSEN2 antibodyPSN2_HUMAN antibodyPSNL2 antibodyPSNL2 antibodySTM-2 antibodySTM2 antibodySTM2 antibody

  see all

Anti-Presenilin 2 antibody [APS 21] images

• 

  Western blot - Presenilin 2 antibody [APS 21] (ab15548)
  All lanes : Anti-Presenilin 2 antibody [APS 21] (ab15548)
  
  Lane 1 : Cell lysate prepared from control siRNA treated Mouse C2C12 myoblasts.
  Lane 2 : Cell lysates prepared from PS2 siRNA treated Mouse C2C12 myoblasts.
  
  Secondary
  HRP-conjugated secondary antibody was used for visualisation by chemiluminescence.

  Image from Ono Y et al, J Cell Sci. 2009 Dec 15;122(Pt 24):4427-38. Epub 2009 Nov 17, Fig 7.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Presenilin 2 antibody [APS 21](ab15548)
  Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Presenilin 2 ab15548 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.