Anti-Presenilin 2 antibody [APS 26] (ab15549)

Overview

• Product nameAnti-Presenilin 2 antibody [APS 26]See all Presenilin 2 primary antibodies ...
• Description
  Mouse monoclonal [APS 26] to Presenilin 2
• SpecificityNo cross-reactivity is seen with presenilin 1.
• Tested applicationsICC/IF, IP, WB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Cow
• Immunogen

  Synthetic peptide: CLPYDPEMEEDSYDSFGEP, corresponding to amino acids 317-334 of Human Presenilin 2 with N-terminally added cysteine.

  Run BLAST with Run BLAST with
• Positive controlRAW cells.

Properties

• FormLiquid
• Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, 1mg/ml BSA
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberAPS 26
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Proteases

  • Neuroscience
  • Neurology process
  • Notch Pathway

Applications

Target

• FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
• Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
• Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
  Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
• Sequence similaritiesBelongs to the peptidase A22A family.
• DomainThe PAL motif is required for normal active site conformation.
• Post-translational
  modificationsHeterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
  Phosphorylated on serine residues.
• Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.

Target information above from: UniProt accession P49810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282010 Cow
  • Entrez Gene: 5664 Human
  • Entrez Gene: 19165 Mouse
  • Entrez Gene: 81751 Rat
  • Omim: 600759 Human
  • SwissProt: Q9XT96 Cow
  • SwissProt: P49810 Human
  • SwissProt: Q61144 Mouse

  • SwissProt: O88777 Rat
  • Unigene: 25363 Human
  • Unigene: 330850 Mouse
  • Unigene: 11045 Rat

  see all

• Alternative names
  AD 4 antibodyAD 4 antibodyAD3L antibody

  AD3L antibodyAD3LP antibodyAD3LP antibodyAD4 antibodyAD4 antibodyAD5 antibodyAD5 antibodyAlzheimer disease 4 antibodyAlzheimer disease 4 antibodyAlzheimer Disease Familial Type 4 antibodyAlzheimer Disease Familial Type 4 antibodyAlzheimer's disease 3 like antibodyAlzheimer's disease 3 like antibodyCMD1V antibodyE5 1 antibodyE5 1 antibodyE5-1 antibodyOTTHUMP00000035671 antibodyOTTHUMP00000035672 antibodyOTTHUMP00000228286 antibodyOTTHUMP00000228288 antibodyPresenilin 2 (Alzheimer disease 4) antibodyPresenilin 2 antibodyPresenilin-2 CTF subunit antibodyPS 2 antibodyPS 2 antibodyPS-2 antibodyPS2 antibodyPS2 antibodyPSEN 2 antibodyPSEN 2 antibodyPSEN2 antibodyPSEN2 antibodyPSN2_HUMAN antibodyPSNL2 antibodyPSNL2 antibodySTM-2 antibodySTM2 antibodySTM2 antibody

  see all

Anti-Presenilin 2 antibody [APS 26] images

• 

  Immunocytochemistry/ Immunofluorescence - Presenilin 2 antibody [APS 26] (ab15549)
  IF staining PS2 using ab15549 
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Presenilin 2 antibody [APS 26](ab15549)
  Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 2 ab15549 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.