RabMAb

Anti-Presenilin 2 antibody [EP1515Y] (ab51249)

Overview

  • Product nameAnti-Presenilin 2 antibody [EP1515Y]
    See all Presenilin 2 primary antibodies
  • Description
    Rabbit monoclonal [EP1515Y] to Presenilin 2
  • Tested applicationsWB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus of human Presenilin 2.

  • Positive control
    • PC-12 cell lysate or human kidney tissue. ICC/IF: HepG2, JAR and A-673 cells.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab51249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).
IHC-P Use at an assay dependent dilution.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
    • Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
    • Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
      Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similaritiesBelongs to the peptidase A22A family.
    • DomainThe PAL motif is required for normal active site conformation.
    • Post-translational
      modifications
      Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
      Phosphorylated on serine residues.
    • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AD 3L antibody
      • AD 3LP antibody
      • AD 4 antibody
      • AD 5 antibody
      • AD3L antibody
      • AD3LP antibody
      • AD4 antibody
      • AD5 antibody
      • Alzheimer disease 4 antibody
      • Alzheimer disease familial type 4 antibody
      • CMD1V antibody
      • E5 1 antibody
      • E5-1 antibody
      • EC 3.4.23. antibody
      • OTTHUMP00000035671 antibody
      • OTTHUMP00000035672 antibody
      • OTTHUMP00000228286 antibody
      • OTTHUMP00000228288 antibody
      • Presenilin 2 (Alzheimer disease 4) antibody
      • Presenilin 2 antibody
      • Presenilin-2 CTF subunit antibody
      • Presenilin2 antibody
      • PS 2 antibody
      • PS-2 antibody
      • PS2 antibody
      • PSEN 2 antibody
      • Psen2 antibody
      • PSN2_HUMAN antibody
      • PSNL 2 antibody
      • PSNL2 antibody
      • STM 2 antibody
      • STM-2 antibody
      • STM2 antibody
      see all

    Anti-Presenilin 2 antibody [EP1515Y] images

    • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized JAR (Human placenta choriocarcinoma cell line) cells labeling Presenilin with ab51249 at 1/250 dilution, followed by Anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on JAR cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab51249 at 1/250 dilution followed by Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

    • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A-673 (Human muscle Ewing's Sarcoma cell line) cells labeling Presenilin with ab51249 at 1/250 dilution, followed by Anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining on A-673 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab51249 at 1/250 dilution followed by Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

       

    • Ab51249 (1:50) staining human Presenilin 2 in human kidney tissue by immunohistochemistry using paraffin embedded tissue.Ab51249 (1:50) staining human Presenilin 2 in human kidney tissue by immunohistochemistry using paraffin embedded tissue.
    • Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/200000 dilution + PC-12 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 50 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/1000 dilution (for 16 hours at 4°C) + Human HeLa cells - whole cell lysate at 30 µg

      Secondary
      An HRP-conjugated Goat anti-rabbit IgG at 1/2000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 50 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      Blocking Step: 5% Milk for 1 hour at 24°C

      See Abreview

    • Presenilin was immunoprecipitated from 1mg of HeLa whole cell extract with ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Input: HeLa whole cell extract 10 µg.

       IP (+): ab51249 IP in HeLa whole cell extract.

       IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Presenilin was immunoprecipitated from 1mg of PC-12 (rat adrenal gland pheochromocytoma) whole cell extract with ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Input: PC-12 whole cell extract 10 µg.

      IP (+): ab51249 IP in PC-12 whole cell extract.

      IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in PC-12 whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    References for Anti-Presenilin 2 antibody [EP1515Y] (ab51249)

    This product has been referenced in:
    • Ludtmann MH  et al. An ancestral non-proteolytic role for presenilin proteins in multicellular development of the social amoeba Dictyostelium discoideum. J Cell Sci 127:1576-84 (2014). Human . Read more (PubMed: 24463814) »
    • Tanveer R  et al. The Endocannabinoid, Anandamide, Augments Notch-1 Signaling in Cultured Cortical Neurons Exposed to Amyloid-ß and in the Cortex of Aged Rats. J Biol Chem 287:34709-21 (2012). WB ; Rat . Read more (PubMed: 22891244) »

    See all 2 Publications for this product

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    The immunogen is located within residues 300 - 350 for this antibody.

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - other (WT AND KO MEF)
    Loading amount 30 µg
    Specification WT AND KO MEF
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 08 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HELA)
    Total protein in input 200 µg
    Specification HELA
    Immuno-precipitation step Protein G
    Username

    Abcam user community

    Verified customer

    Submitted Sep 22 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cultured Cells (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton-X-0.5%
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 14 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HELA)
    Loading amount 30 µg
    Specification HELA
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 30 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"