Anti-Presenilin 2 (phospho S327) antibody [EP2613Y] (ab76114)

Overview

• Product nameAnti-Presenilin 2 (phospho S327) antibody [EP2613Y]See all Presenilin 2 primary antibodies ...
• Description
  Rabbit monoclonal [EP2613Y] to Presenilin 2 (phospho S327)
• Specificityab76114 detects Presenilin 2 phosphorylated on serine 327.
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A phospho specific peptide corresponding to residues surrounding serine 327 of human Presenilin 2

• Positive controlHela cell lysates.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP2613Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Neuroscience
  • Neurology process
  • Notch Pathway

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Proteases

Applications

Target

• FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
• Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
• Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
  Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
• Sequence similaritiesBelongs to the peptidase A22A family.
• DomainThe PAL motif is required for normal active site conformation.
• Post-translational
  modificationsHeterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
  Phosphorylated on serine residues.
• Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.

Target information above from: UniProt accession P49810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5664 Human
  • Entrez Gene: 19165 Mouse
  • Entrez Gene: 81751 Rat
  • Omim: 600759 Human
  • SwissProt: P49810 Human
  • SwissProt: Q61144 Mouse
  • SwissProt: O88777 Rat
  • Unigene: 25363 Human

  • Unigene: 330850 Mouse
  • Unigene: 11045 Rat

  see all

• Alternative names
  AD 3L antibodyAD 3LP antibodyAD 4 antibody

  AD 5 antibodyAD3L antibodyAD3LP antibodyAD4 antibodyAD5 antibodyAlzheimer disease 4 antibodyAlzheimer disease familial type 4 antibodyCMD1V antibodyE5 1 antibodyE5-1 antibodyEC 3.4.23. antibodyOTTHUMP00000035671 antibodyOTTHUMP00000035672 antibodyOTTHUMP00000228286 antibodyOTTHUMP00000228288 antibodyPresenilin 2 (Alzheimer disease 4) antibodyPresenilin 2 antibodyPresenilin-2 CTF subunit antibodyPresenilin2 antibodyPS 2 antibodyPS-2 antibodyPS2 antibodyPSEN 2 antibodyPsen2 antibodyPSN2_HUMAN antibodyPSNL 2 antibodyPSNL2 antibodySTM 2 antibodySTM-2 antibodySTM2 antibody

  see all

Anti-Presenilin 2 (phospho S327) antibody [EP2613Y] images

• 

  Western blot - Presenilin 2 (phospho S327) antibody [EP2613Y] (ab76114)
  All lanes : Anti-Presenilin 2 (phospho S327) antibody [EP2613Y] (ab76114) at 1/5000 dilution
  
  Lane 1 : HeLa cell lysates un-treated
  Lane 2 : HeLa cell lysates treated with AP
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 50 kDa
  Observed band size : 23 kDa (why is the actual band size different from the predicted?)