Anti-Presenilin 2 (phospho S327) antibody [EP2613Y] (ab76114)

Overview

  • Product name
    Anti-Presenilin 2 (phospho S327) antibody [EP2613Y]
    See all Presenilin 2 primary antibodies
  • Description
    Rabbit monoclonal [EP2613Y] to Presenilin 2 (phospho S327)
  • Specificity
    ab76114 detects Presenilin 2 phosphorylated on serine 327.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 327 of human Presenilin 2

  • Positive control
    • Hela cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).
IP 1/10.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
    • Tissue specificity
      Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
    • Involvement in disease
      Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
      Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities
      Belongs to the peptidase A22A family.
    • Domain
      The PAL motif is required for normal active site conformation.
    • Post-translational
      modifications
      Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
      Phosphorylated on serine residues.
    • Cellular localization
      Endoplasmic reticulum membrane. Golgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AD3L antibody
      • AD3LP antibody
      • AD4 antibody
      • AD5 antibody
      • Alzheimer disease 4 antibody
      • CMD1V antibody
      • E5-1 antibody
      • OTTHUMP00000035671 antibody
      • OTTHUMP00000035672 antibody
      • OTTHUMP00000228286 antibody
      • OTTHUMP00000228288 antibody
      • Presenilin 2 (Alzheimer disease 4) antibody
      • Presenilin 2 antibody
      • Presenilin-2 CTF subunit antibody
      • PS-2 antibody
      • PS2 antibody
      • Psen2 antibody
      • PSN2_HUMAN antibody
      • PSNL2 antibody
      • STM-2 antibody
      • STM2 antibody
      see all

    Images

    • All lanes : Anti-Presenilin 2 (phospho S327) antibody [EP2613Y] (ab76114) at 1/5000 dilution

      Lane 1 : HeLa cell lysates un-treated
      Lane 2 : HeLa cell lysates treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 50 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)

    References

    ab76114 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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