Anti-Presenilin 2 (phospho S330) antibody [EP2614] (ab109456)

Overview

• Product nameAnti-Presenilin 2 (phospho S330) antibody [EP2614]See all Presenilin 2 primary antibodies ...
• Description
  Rabbit monoclonal [EP2614] to Presenilin 2 (phospho S330)
• SpecificityThis antibody only detects Presenilin 2 when phosphorylated on Serine 330.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A phospho-specific peptide corresponding to residues surrounding Serine 330 in Human Presenilin 2.

• Positive controlHeLa cell lysates.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at -20ºC.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP2614
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Proteases

  • Neuroscience
  • Neurology process
  • Notch Pathway

Applications

Target

• FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
• Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
• Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
  Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
• Sequence similaritiesBelongs to the peptidase A22A family.
• DomainThe PAL motif is required for normal active site conformation.
• Post-translational
  modificationsHeterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
  Phosphorylated on serine residues.
• Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.

Target information above from: UniProt accession P49810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5664 Human
  • Entrez Gene: 19165 Mouse
  • Entrez Gene: 81751 Rat
  • Omim: 600759 Human
  • SwissProt: P49810 Human
  • SwissProt: Q61144 Mouse
  • SwissProt: O88777 Rat
  • Unigene: 25363 Human

  • Unigene: 330850 Mouse
  • Unigene: 11045 Rat

  see all

• Alternative names
  AD3L antibodyAD3LP antibodyAD4 antibody

  AD5 antibodyAlzheimer disease 4 antibodyCMD1V antibodyE5-1 antibodyOTTHUMP00000035671 antibodyOTTHUMP00000035672 antibodyOTTHUMP00000228286 antibodyOTTHUMP00000228288 antibodyPresenilin 2 (Alzheimer disease 4) antibodyPresenilin 2 antibodyPresenilin-2 CTF subunit antibodyPS-2 antibodyPS2 antibodyPsen2 antibodyPSN2_HUMAN antibodyPSNL2 antibodySTM-2 antibodySTM2 antibody

  see all

Anti-Presenilin 2 (phospho S330) antibody [EP2614] images

• 

  Western blot - Presenilin 2 (phospho S330) antibody [EP2614] (ab109456)
  All lanes : Anti-Presenilin 2 (phospho S330) antibody [EP2614] (ab109456) at 1/5000 dilution
  
  Lane 1 : HeLa cell lysate untreated with with Alkaline Phosphatase
  Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 50 kDa