Overview
- Broad range: 10-175 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye.
- Sharp bands
Properties
Concentration information loading...-
Alternative names
- Molecular weight markerPrism protein ladderProtein Ladder
- SDS PAGE protein ladder prestainedWB prestained protein ladderWestern blot prestained protein ladder
see all
Applications
Our Abpromise guarantee covers the use of ab115832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 - 5ul. |
| SDS-PAGE | SDS-PAGE: Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 ul per well for mini-gels and 10 ul for larger gels. |
Prism Protein Ladder (10-175 kDa) images
-
SDS-PAGE - Prism Protein Ladder (10-175 kDa) (ab115832)SDS-PAGE with ab115832. Gel 1: Tris-Glycine 15%, Gel 2: Bis-Tris 4-12% MOPS buffer and Gel 3: Bis-Tris 4-12% MES Buffer.Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
Protocols
References for Prism Protein Ladder (10-175 kDa) (ab115832)
ab115832 has not yet been referenced specifically in any publications.
