Prism Ultra Protein Ladder (10-245 kDa) (ab116028)

Overview

  • Product namePrism Ultra Protein Ladder (10-245 kDa)
    See all Prestained Protein Ladder reagents
  • Tested applicationsSDS-PAGE, WBmore details
  • General notes

    ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

    ab116028 is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

    Key product features:
    • Broad range: 10-245 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 - 2.5µl

    Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Properties

  • Alternative names
    • Molecular weight marker
    • Prism protein ladder
    • Protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder
    see all

Applications

Our Abpromise guarantee covers the use of ab116028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
WB Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

Prism Ultra Protein Ladder (10-245 kDa) images

  • Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer.Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

References for Prism Ultra Protein Ladder (10-245 kDa) (ab116028)

ab116028 has not yet been referenced specifically in any publications.

Product Wall

Approximately 0.1˜0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25℃), 2 % SDS, 3.6 M Urea, and 15 % (v/v) Glycerol).

So for a 500ul vial, each protein's concentration is approximately 0.05 - 0.2mg/500ul. For a 5ul l...

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Merci pour votre appel.

Je peux vous confirmer que nos marqueurs de poids moléculaires, ab116027, ab116028 ou ab116029 ne contiennent aucune protéine avecun His tag.

Je tiens aussi à vous signaler, que des fois,...

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Thank you for contacting us.


I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by...

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Thanks for sending along the western blot.

You might be detecting a specific band in there but I agree there are too many non specific bands to be certain. Because of this high background I'm happy to offer a replacement, credit or refund if ...

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Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"