Anti-Pro + Mature Surfactant Protein B antibody (ab40876)

Overview

  • Product name
    Anti-Pro + Mature Surfactant Protein B antibody
  • Description
    Rabbit polyclonal to Pro + Mature Surfactant Protein B
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Cow, Human
  • Immunogen

    Full length native SP-B protein (purified) (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab40876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 42 kDa. (reduced or non-reduced samples).
ELISA 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • Alternative names
    • PSP B antibody
    • PSPB antibody
    • Pulmonary surfactant apoprotein PSP-B antibody
    • SFTPB antibody
    • SP B antibody
    • SP-B antibody
    • SPB antibody
    • surfactant protein B antibody
    • Surfactant, pulmonary-associated protein B antibody
    see all

Images

  • Adult mouse lung was embedded in paraffin and stained with ab40876 (1/500 dilution).
  • All lanes : Anti-Pro + Mature Surfactant Protein B antibody (ab40876) at 1/5000 dilution

    Lane 1 : Adult mouse lung homogenate
    Lane 2 : Human lung homogenate
    Lane 3 : Surfactant Protein B peptide
    Lane 4 : HEK-293T lysate


    Predicted band size : 42 kDa

References

This product has been referenced in:
  • Bartolák-Suki E  et al. Optimization of Variable Ventilation for Physiology, Immune Response and Surfactant Enhancement in Preterm Lambs. Front Physiol 8:425 (2017). Read more (PubMed: 28690548) »
  • Khoshgoo N  et al. MicroRNA-200b regulates distal airway development by maintaining epithelial integrity. Sci Rep 7:6382 (2017). Read more (PubMed: 28743913) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (amniotic epithelial cells)
Permeabilization
Yes - Permeabilization Buffer
Gating Strategy
Epithelial cells
Specification
amniotic epithelial cells
Fixation
Formaldehyde
Username

Ms. Angela Lemke

Verified customer

Submitted Jan 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Liver [surfactant protein B])
Loading amount
100 µg
Specification
Liver [surfactant protein B]
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. SeongHoon Cho

Verified customer

Submitted Nov 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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