Anti-Procollagen Type 1 antibody [2Q576] (ab64409)

Overview

  • Product nameAnti-Procollagen Type 1 antibody [2Q576]See all Procollagen Type 1 primary antibodies ...
  • Description
    Rat monoclonal [2Q576] to Procollagen Type 1
  • Specificityab64409 recognizes Human procollagen I. Labels the amino-terminal pro-peptide. Localized intracellularly in cells producing pro-collagen I. Does not stain mature collagen fibers in tissue.
  • Tested applicationsFlow Cyt, ICC, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Human procollagen I.

  • Positive control
    • Human skin

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Tissue culture supernatant
  • PurityTissue culture supernatant
  • Clonality Monoclonal
  • Clone number2Q576
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab64409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21966390
ICC Use at an assay dependent concentration.
IHC-P 1/100 - 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend antigen retrieval with 1% trypsin for 20 minutes at room temperature.
IHC-Fr 1/10000 - 1/50000.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceCollagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Procollagen type 1 is mainly synthesized by osteoblasts and, after cleavage of the N- and C-terminal extension peptides, is utilized for collagen fibril deposition in the osteoid tissue.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • Alpha 1 type I collagen antibody
      • Alpha 2 type I collagen antibody
      • COL1A1 antibody
      • COL1A2 antibody

    Anti-Procollagen Type 1 antibody [2Q576] images

    • ab64409 staining Procollagen Type 1 in Human skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.25% serum for 15 minutes at 25°C ; enzymatic antigen retrieval using Proteinase K was performed. Samples were incubated with primary antibody (1/800 in serum free antibody diluent) for 30 minutes at 25°C. A Biotin-conjugated Rabbit anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • ab64409 staining Procollagen Type 1 in Pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and permeabilized with Triton X100. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. Ab98387 (A DyLight®550-conjugated Goat anti-rat IgG polyclonal; 1/500) was used as the secondary antibody.

      See Abreview

    • ab64409 staining Procollagen Type 1 (green) in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/100) was used as the secondary antibody. Red - Immune Marker, Blue - DAPI.

      See Abreview

    References for Anti-Procollagen Type 1 antibody [2Q576] (ab64409)

    This product has been referenced in:
    • Karén J  et al. Effects of the histone deacetylase inhibitor valproic Acid on human pericytes in vitro. PLoS One 6:e24954 (2011). Flow Cyt ; Human . Read more (PubMed: 21966390) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample Pig Tissue sections (Skeletal Muscle)
    Specification Skeletal Muscle
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 04 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin 1% 20m RT
    Sample Human Tissue sections (Skeletal Muscle)
    Specification Skeletal Muscle
    Permeabilization No
    Fixative Methacarn
    Username

    Abcam user community

    Verified customer

    Submitted Oct 18 2013

    DISCOUNT CODE: *
    Expiration date: *

    I am very pleased to hear you would like to accept our offer and test ab66409 in ELISA. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an...

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    Application Immunohistochemistry (Frozen sections)
    Sample Pig Tissue sections (Epithelial)
    Specification Epithelial
    Fixative Paraformaldehyde
    Permeabilization Yes - .1% Triton X100
    Username

    Mr. Daniel Holod-Betz

    Verified customer

    Submitted Apr 11 2012

    Thank you for your call today and for keeping me updated about the progress with this antibody.

    As we discussed, I would recommend trying a dilution around 1:600 for aone hourincubation, or 1:800 overnight at 4C. You can also try increasing t...

    Read More

    Thanks for your call today and for letting us know about the trouble with ab64409.

    Please keep me updated about any new results using the antibody as we discussed.

    Let me know if you have any questions or if there is anything else t...

    Read More
    Application Immunocytochemistry
    Sample Mouse Cultured Cells (Tracheal Fibroblast)
    Specification Tracheal Fibroblast
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 14 2012


    I am very pleased to hear you would like to accept our offer and test ab10559 and ab64409 in samples.This code will give you 1 freeprimary antibodybefore the expiration date for each discount code. To redeem this offer, please submit an Abreview f...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (skin)
    Specification skin
    Fixative Formaldehyde
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin 0,1%
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 03 2012

    DISCOUNT CODE: XXXXXXXX Expiration date: 14 April 2012 I am very pleased to hear you would like to accept our offer and test ab64409 in Mouse. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, ple...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"