Anti-Procollagen Type 1 antibody [2Q576] (ab64409)

Overview

• Product nameAnti-Procollagen Type 1 antibody [2Q576]
• Description
  Rat monoclonal [2Q576] to Procollagen Type 1
• Specificityab64409 recognizes Human procollagen I. Labels the amino-terminal pro-peptide. Localized intracellularly in cells producing pro-collagen I. Does not stain mature collagen fibers in tissue.
• Tested applicationsFlow Cyt, ICC, IHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Human, Pig
  
• Immunogen

  Human procollagen I.

• Positive controlHuman skin

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Tissue culture supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone number2Q576
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• RelevanceCollagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Procollagen type 1 is mainly synthesized by osteoblasts and, after cleavage of the N- and C-terminal extension peptides, is utilized for collagen fibril deposition in the osteoid tissue.
• Cellular localizationSecreted
• Database links
  • Entrez Gene: 1278 Human
  • Entrez Gene: 1277 Human
  • SwissProt: P02452 Human
  • SwissProt: P08123 Human

• Alternative names
  Alpha 1 type I collagen antibodyAlpha 2 type I collagen antibodyCOL1A1 antibodyCOL1A2 antibody

Anti-Procollagen Type 1 antibody [2Q576] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Procollagen Type 1 antibody [2Q576] (ab64409)This image is courtesy of an Abreview submitted by Ross Benik
  ab64409 staining Procollagen Type 1 in Human skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.25% serum for 15 minutes at 25°C ; enzymatic antigen retrieval using Proteinase K was performed. Samples were incubated with primary antibody (1/800 in serum free antibody diluent) for 30 minutes at 25°C. A Biotin-conjugated Rabbit anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

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• 

  Immunohistochemistry (Frozen sections) - Anti-Procollagen Type 1 antibody [2Q576] (ab64409)This image is courtesy of an Abreview submitted by Daniel Holod-Betz
  ab64409 staining Procollagen Type 1 in Pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and permeabilized with Triton X100. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. Ab98387 (A DyLight^®550-conjugated Goat anti-rat IgG polyclonal; 1/500) was used as the secondary antibody.

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