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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Human procollagen I.
Our Abpromise guarantee covers the use of ab64409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21966390|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/100 - 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend antigen retrieval with 1% trypsin for 20 minutes at room temperature.|
|IHC-Fr||1/10000 - 1/50000.|
ab64409 staining Procollagen Type 1 in Pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and permeabilized with Triton X100. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. Ab98387 (A goat anti-rat DyLight®550 (IgG; 1/500)) was used as the secondary antibody.
ab64409 staining Procollagen Type 1 (green) in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/100) was used as the secondary antibody. Red - Immune Marker, Blue - DAPI.
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