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ab50667 has been referenced in 3 publications.
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Lane 1: Positve control — Western blot: ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.
Lane 2: Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody.
Lane 3: Negative control — non-relevant antibody was used for IP. Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody
ab50667 detecting Profilin 1 in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The cell population was gated on platelets. The primary antibody was diluted 1/200 in 2% BSA in PBS and incubated with the sample for 18 hours at 4ºC. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody.
Abbreviations in the figure:
US Unstained, Red Peak;
IgG Rb: IgG Rabbit (Isotype Control), Blue Peak;
Profilin: Profilin 1, Green peak.
This image is courtesy of an Abreview by Mahesh Shivananjappa.
Anti-Profilin 1 antibody - Carboxyterminal end (ab50667) at 1/1000 dilution + Human platelet lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Image courtesy of Mahesh Shivananjappa, United States
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